发明公开
EP1490514A2 SINGLE PRIMER ISOTHERMAL NUCLEIC ACID AMPLIFICATION-ENHANCED ANALYTE DETECTION AND QUANTIFICATION
审中-公开
BY ISOTHERME单个引物,核酸扩增改进的检测和分析物的定量
- 专利标题: SINGLE PRIMER ISOTHERMAL NUCLEIC ACID AMPLIFICATION-ENHANCED ANALYTE DETECTION AND QUANTIFICATION
- 专利标题(中): BY ISOTHERME单个引物,核酸扩增改进的检测和分析物的定量
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申请号: EP03718172.4申请日: 2003-03-31
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公开(公告)号: EP1490514A2公开(公告)日: 2004-12-29
- 发明人: KURN, Nurith , DAFFORN, Geoffrey, A.
- 申请人: NuGEN Technologies, Inc.
- 申请人地址: 821 Industrial Road, Unit A San Carlos, CA 94070 US
- 专利权人: NuGEN Technologies, Inc.
- 当前专利权人: NuGEN Technologies, Inc.
- 当前专利权人地址: 821 Industrial Road, Unit A San Carlos, CA 94070 US
- 代理机构: Roques, Sarah Elizabeth
- 优先权: US368628P 20020329
- 国际公布: WO2003083435 20031009
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; C12P21/08 ; C07H21/02 ; C07H21/04 ; G01N33/53
摘要:
The present invention provides novel methods of indirect analyte detection and quantification through amplification of oligonucleotide template attached to binding partners for analytes by nucleic acid amplification utilizing isothermal, single primer linear nucleic acid amplification methods. Methods of binding of binding partner that is attached to an oligonucleotide template to analyte, then amplifying at least a portion of the oligonucleotide template using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. Methods for detecting and quantifying amplification products are also provided. The invention further provides compositions and kits for practicing said methods.
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