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1.发明公开SINGLE PRIMER ISOTHERMAL NUCLEIC ACID AMPLIFICATION-ENHANCED ANALYTE DETECTION AND QUANTIFICATION 审中-公开BY ISOTHERME单个引物，核酸扩增改进的检测和分析物的定量
公开(公告)号：EP1490514A2
公开(公告)日：2004-12-29
申请号：EP03718172.4
申请日：2003-03-31
CPC分类号： C12Q1/6804 , C12Q1/6865 , C12Q2563/179 , C12Q2525/143 , C12Q2525/121 , C12Q2521/319 , C12Q2537/1373 , C12Q2521/313
摘要： The present invention provides novel methods of indirect analyte detection and quantification through amplification of oligonucleotide template attached to binding partners for analytes by nucleic acid amplification utilizing isothermal, single primer linear nucleic acid amplification methods. Methods of binding of binding partner that is attached to an oligonucleotide template to analyte, then amplifying at least a portion of the oligonucleotide template using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. Methods for detecting and quantifying amplification products are also provided. The invention further provides compositions and kits for practicing said methods.
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2.发明公开METHODS FOR ANALYSIS OF NUCLEIC ACID METHYLATION STATUS AND METHODS FOR FRAGMENTATION, LABELING AND IMMOBILIZATION OF NUCLEIC ACIDS 审中-公开方法核酸分析甲基化和碎片化状态的方法，标志和核酸的固定化
公开(公告)号：EP1711591A2
公开(公告)日：2006-10-18
申请号：EP04815722.6
申请日：2004-12-29
IPC分类号： C12N1/00
CPC分类号： C12Q1/6827 , C12P19/34 , C12Q2525/119 , C12Q2521/531
摘要： The invention relates to methods for analysis of nucleic acid methylation status, and fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.
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3.发明公开
公开(公告)号：EP1573056A2
公开(公告)日：2005-09-14
申请号：EP03771533.1
申请日：2003-05-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , C12Q1/6806 , C12Q1/6837 , C12Q2525/119 , C12Q2521/531 , C12Q2525/101 , C12Q2523/107
摘要： The invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.
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