发明授权
- 专利标题： HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD
- 专利标题（中）： 具有高吞吐量诱变筛选过程
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申请号： EP03744218.3申请日： 2003-03-05
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公开(公告)号： EP1543117B1公开(公告)日： 2009-09-30
- 发明人: BOTT, Richard, R. , KELLIS, James, T., Jr. , MORRISON, Thomas, B.
- 申请人： GENENCOR INTERNATIONAL, INC.
- 申请人地址： 925 Page Mill Road Palo Alto, California 94304 US
- 专利权人： GENENCOR INTERNATIONAL, INC.
- 当前专利权人： GENENCOR INTERNATIONAL, INC.
- 当前专利权人地址： 925 Page Mill Road Palo Alto, California 94304 US
- 代理机构： Kiddle, Simon John
- 优先权： US91912 20020305; US92227 20020305; US362372P 20020305; US403921P 20020815
- 国际公布： WO2003076580 20030918
- 主分类号： C12N9/20
- IPC分类号： C12N9/20
摘要：
The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.
公开/授权文献
- EP1543117A2 HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD 公开/授权日：2005-06-22
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