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    HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD
    2.
    发明授权
    HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD 有权
    具有高吞吐量诱变筛选过程

    公开(公告)号：EP1543117B1

    公开(公告)日：2009-09-30

    申请号：EP03744218.3

    申请日：2003-03-05

    申请人： GENENCOR INTERNATIONAL, INC.

    发明人： BOTT, Richard, R. ,  KELLIS, James, T., Jr. ,  MORRISON, Thomas, B.

    IPC分类号： C12N9/20

    CPC分类号： C12Y301/01074 ,  C12N9/18

    摘要： The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.

    BLEACHING COMPOSITIONS COMPRISING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS
    5.
    发明授权
    BLEACHING COMPOSITIONS COMPRISING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 有权
    漂白剂组合物含有多种取代的蛋白酶变

    公开(公告)号：EP1025194B2

    公开(公告)日：2012-01-04

    申请号：EP98955079.3

    申请日：1998-10-23

    申请人： GENENCOR INTERNATIONAL, INC.

    发明人： GHOSH, Chanchal, Kumar ,  BAECK, Andre, Cesar ,  OHTANI, Ryohei ,  BUSCH, Alfred ,  SHOWELL, Michael, Stanford ,  SCHELLENBERGER, Volker ,  KELLIS, James, T., Jr. ,  PAECH, Christian ,  NADHERNY, Joanne ,  NAKI, Donald, P. ,  COLLIER, Katherine, D. ,  CALDWELL, Robert, M. ,  POULOSE, Ayrookaran, J.

    IPC分类号： C12N9/54

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.

    HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD
    9.
    发明公开
    HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD 有权
    具有高吞吐量诱变筛选过程

    公开(公告)号：EP1543117A2

    公开(公告)日：2005-06-22

    申请号：EP03744218.3

    申请日：2003-03-05

    申请人： GENENCOR INTERNATIONAL, INC.

    发明人： BOTT, Richard, R. ,  KELLIS, James, T., Jr. ,  MORRISON, Thomas, B.

    IPC分类号： C12N9/20

    CPC分类号： C12Y301/01074 ,  C12N9/18

    摘要： The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.