发明公开
- 专利标题： IMPROVED LUCIFERASE-BASED ASSAYS
- 专利标题（中）： 改进ASSAYS荧光素酶为基础
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申请号： EP03800272.1申请日： 2003-12-23
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公开(公告)号： EP1588143A2公开(公告)日： 2005-10-26
- 发明人: HAWKINS, Erika , CALI, James, J. , HO, Samuel, Kin, Sang , O'BRIEN, Martha , SOMBERG, Richard , BULLEIT, Robert, F. , WOOD, Keith, V.
- 申请人： PROMEGA CORPORATION
- 申请人地址： 2800 Woods Hollow Road Madison, Wisconsin 53711 US
- 专利权人： PROMEGA CORPORATION
- 当前专利权人： PROMEGA CORPORATION
- 当前专利权人地址： 2800 Woods Hollow Road Madison, Wisconsin 53711 US
- 代理机构： Webber, Philip Michael
- 优先权： US436173P 20021223; US444264P 20030131; US447334P 20030213
- 国际公布： WO2004059294 20040715
- 主分类号： G01N1/00
- IPC分类号： G01N1/00
摘要：
A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
公开/授权文献
- EP1588143B1 IMPROVED LUCIFERASE-BASED ASSAYS 公开/授权日：2014-03-05
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