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公开(公告)号：EP2959010B1
公开(公告)日：2020-07-29
申请号：EP14709480.9
申请日：2014-02-21
申请人： Promega Corporation
发明人： VALLEY, Michael, P. , CALI, James, J. , BINKOWSKI, Brock , EGGERS, Christopher, Todd , WOOD, Keith, V.
IPC分类号： C12Q1/66
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公开(公告)号：EP1588143B1
公开(公告)日：2014-03-05
申请号：EP03800272.1
申请日：2003-12-23
申请人： PROMEGA CORPORATION
发明人： HAWKINS, Erika , CALI, James, J. , HO, Samuel, Kin, Sang , O'BRIEN, Martha , SOMBERG, Richard , BULLEIT, Robert, F. , WOOD, Keith, V.
CPC分类号： C12Q1/66
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公开(公告)号：EP1588143A2
公开(公告)日：2005-10-26
申请号：EP03800272.1
申请日：2003-12-23
申请人： PROMEGA CORPORATION
发明人： HAWKINS, Erika , CALI, James, J. , HO, Samuel, Kin, Sang , O'BRIEN, Martha , SOMBERG, Richard , BULLEIT, Robert, F. , WOOD, Keith, V.
IPC分类号： G01N1/00
CPC分类号： C12Q1/66
摘要： A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
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公开(公告)号：EP2751089B1
公开(公告)日：2018-11-14
申请号：EP12772562.0
申请日：2012-08-31
申请人： Promega Corporation
发明人： BENINK, Helene A. , CALI, James, J. , DUELLMAN, Sarah , KLAUBERT, Dieter , LEIPPE, Donna , O'BRIEN, Martha , SHULTZ, John , VIDUGIRIENE, Jolanta , ZHOU, Wenhui , SOBOL, Mary
IPC分类号： C07D497/10 , C07F9/6558 , C07F9/6561 , C12Q1/32 , G01N21/76 , G01N33/50 , G01N33/573 , C07D277/68 , C07D487/04 , C12Q1/66 , G01N33/58
CPC分类号： C12Q1/32 , C07D277/68 , C07D417/04 , C07D487/04 , C07D493/10 , C07D497/10 , C07F9/65583 , C07F9/6561 , C12Q1/66 , G01N21/763 , G01N33/5038 , G01N33/5735 , G01N33/582
摘要： The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.
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5.发明公开BIOLUMINESCENT ASSAYS USING CYANOBENZOTHIAZOLE COMPOUNDS 有权微生物酶联免疫吸附测定法（MIT CYANOBENZOTHIAZOLVERBINDUNGEN）
公开(公告)号：EP2545041A1
公开(公告)日：2013-01-16
申请号：EP11709845.9
申请日：2011-03-11
申请人： Promega Corporation
IPC分类号： C07D277/68 , C07D417/12 , C07K5/10 , C12Q1/25 , C12Q1/26 , C12Q1/34 , C12Q1/48 , C12Q1/66 , G01N21/76
CPC分类号： C07D277/68 , C07D417/12 , C07K5/1005 , C07K5/1021 , G01N21/763 , G01N33/582
摘要： The invention provides methods that employ derivatives of 2-cyano-6-hydoxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.
摘要翻译： 本发明提供了使用2-氰基-6-羟基 - 或2-氰基-6-氨基 - 苯并噻唑衍生物的方法，例如在生物发光反应中。 本发明还提供了用于检测或确定分子和/或酶的存在，这种分子的调节活性和/或这些酶的活性的方法。 这些方法适用于高吞吐量格式。
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公开(公告)号：EP2331517A1
公开(公告)日：2011-06-15
申请号：EP09789278.0
申请日：2009-09-09
申请人： Promega Corporation
CPC分类号： C07D277/68 , C07K5/101 , C07K5/1021 , C12Q1/26 , C12Q1/34 , C12Q1/37 , C12Q1/48 , C12Q1/66 , C12Q2326/00 , G01N21/763
摘要： The invention provides methods that employ derivatives of 2-cyano-6-hydoxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. Also provided are novel compounds that can be used in the methods. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.
摘要翻译： 本发明提供了例如在生物发生反应中使用2-氰基-6-羟基 - 或2-氰基-6-氨基 - 苯并噻唑的衍生物的方法。 还提供了可用于该方法的新化合物。 本发明还提供了用于检测或确定分子和/或酶的存在，这些分子的调节剂活性和/或这些酶的活性的方法。 这些方法适用于高通量格式。
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7.发明公开
公开(公告)号：EP4306649A2
公开(公告)日：2024-01-17
申请号：EP23213374.4
申请日：2014-02-21
申请人： Promega Corporation
发明人： VALLEY, Michael, P. , CALI, James, J. , BINKOWSKI, Brock , EGGERS, Christopher, Todd , WOOD, Keith, V.
IPC分类号： C12Q1/66
摘要： Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.
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公开(公告)号：EP3099683B1
公开(公告)日：2020-08-05
申请号：EP15705425.5
申请日：2015-01-29
申请人： Promega Corporation
发明人： MUSTAFA, Dana , ZHOU, Wenhui , MEISENHEIMER, Poncho , DUELLMAN, Sarah , MA, Dongping , CALI, James, J.
IPC分类号： C07D417/12 , C07D487/04 , C07D417/04 , C07D417/14 , C07J9/00 , C07J19/00 , C07D493/10 , C12Q1/66 , G01N33/58
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公开(公告)号：EP2900833A1
公开(公告)日：2015-08-05
申请号：EP13841100.4
申请日：2013-09-26
申请人： Promega Corporation
CPC分类号： C12Q1/66 , G01N33/5005
摘要： Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response.
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10.发明公开
公开(公告)号：EP4306649A3
公开(公告)日：2024-04-03
申请号：EP23213374.4
申请日：2014-02-21
申请人： Promega Corporation
发明人： VALLEY, Michael, P. , CALI, James, J. , BINKOWSKI, Brock , EGGERS, Christopher, Todd , WOOD, Keith, V.
IPC分类号： C12Q1/66
摘要： Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.
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