发明公开
- 专利标题: QUANTITATIVE METHOD FOR DETECTING YESSOTOXINS IN FISH PRODUCTS BASED ON THE ACTIVATION CAUSED BY THE TOXIN IN CELLULAR PHOSPHODIESTERASE AND THERAPEUTIC USEFULNESS OF SAID ACTIVATION
- 专利标题(中): 定量方法基于毒素诱导激活蜂窝磷酸YESSOTOXINEN鱼制品的检测
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申请号: EP04742071.6申请日: 2004-07-21
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公开(公告)号: EP1647599A2公开(公告)日: 2006-04-19
- 发明人: Botana Lopez, L.M., Univ. Santiago de Compostela , Alfonso Rancano, A., Univ. Santiago de Compostela , Pazos Guldris, M.J., Univ. Santiago de Compostela , Rodrigeuz Vieytes, M., Univ. Sant. de Compostela , Loza Garcia, M.I., Univ. Santiago de Compstela , Vieites Baptista De Sousa, Juan Manuel
- 申请人: UNIVERSIDADE DE SANTIAGO DE COMPOSTELA
- 申请人地址: CITT - Edificio CACTUS, Campus Sur 15782 Santiago de Compostela ES
- 专利权人: UNIVERSIDADE DE SANTIAGO DE COMPOSTELA
- 当前专利权人: UNIVERSIDADE DE SANTIAGO DE COMPOSTELA
- 当前专利权人地址: CITT - Edificio CACTUS, Campus Sur 15782 Santiago de Compostela ES
- 代理机构: Carvajal y Urquijo, Isabel
- 优先权: ES200301773 20030725
- 国际公布: WO2005012543 20050210
- 主分类号: C12Q1/44
- IPC分类号: C12Q1/44 ; G01N21/64 ; A61K31/35 ; A61P11/06 ; A61P37/08 ; A61P35/00
摘要:
The present invention relates to a quantitative method for detecting yessotoxins in fishery products based on the activation the toxin produces on cellular phosphodiesterases and the therapeutic use of this activation. The cellular target of yessotoxin (YTX) and its analogs is the activation of phosphodiesterases (PDEs). The PDEs-YTX bond produces a measurable signal. The bond can be quantified by means of an affinity biosensor or by fluorescence. The biosensor detects biomolecular interactions and allows determining the presence of YTX due to its interaction with PDEs. The variations in the degradation rate of the fluorescent derivative anthraniloyl-cAMP are determined by means of plate fluorescence. The rate at which the PDEs degrade this molecule increases in the presence of YTX. YTX inhibits immunological activation of mastocytes in rats and induces a cytotoxic effect in human hepatocarcinoma cells, which implies two therapeutic uses of YTXs as an antiallergic and antitumor compound.
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