公开(公告)号：EP1647599B1

公开(公告)日：2008-04-30

申请号：EP04742071.6

申请日：2004-07-21

申请人： UNIVERSIDADE DE SANTIAGO DE COMPOSTELA

发明人： Botana Lopez, L.M., Univ. Santiago de Compostela ,  Alfonso Rancano, A., Univ. Santiago de Compostela ,  Pazos Guldris, M.J., Univ. Santiago de Compostela ,  Rodrigeuz Vieytes, M., Univ. Sant. de Compostela ,  Loza Garcia, M.I., Univ. Santiago de Compstela ,  Vieites Baptista De Sousa, Juan Manuel

IPC分类号： C12Q1/44 ,  G01N21/64 ,  A61K31/35 ,  A61P11/06 ,  A61P37/08 ,  A61P35/00

CPC分类号： C12Q1/44 ,  A61K31/35

摘要： The invention relates to a quantitative method for detecting yessotoxins in fish products based on the activation caused by the toxin in cellular phosphodiesterase and therapeutic usefulness of said activation. The cellular target of yessotoxin (YTX) and analogues is the activation of phosphodiesterases (PDEs). The PDEs-YTX union emits a measurable signal. The union can be quantified by means of an affinity biosensor or fluorescence. The biosensor detects bimolecular interactions and makes it possible to determine the presence of YTX due to its interaction with PDEs. Plate fluorescence makes it possible to determine variations in the speed of degradation of fluorescent derivative anthranyloil-AMPc. The speed with which the PDEs degrade this molecule increases in the presence of YTX. The YTX inhibits the immunological activation of rat mastocytese and induces a cytotoxic effect in human hepatocarcinoma cells, which provides two therapeutic utilities of YTXs as anti-allergic and anti-tumoral compound.

公开(公告)号：EP1647599A2

公开(公告)日：2006-04-19

申请号：EP04742071.6

申请日：2004-07-21

申请人： UNIVERSIDADE DE SANTIAGO DE COMPOSTELA

发明人： Botana Lopez, L.M., Univ. Santiago de Compostela ,  Alfonso Rancano, A., Univ. Santiago de Compostela ,  Pazos Guldris, M.J., Univ. Santiago de Compostela ,  Rodrigeuz Vieytes, M., Univ. Sant. de Compostela ,  Loza Garcia, M.I., Univ. Santiago de Compstela ,  Vieites Baptista De Sousa, Juan Manuel

IPC分类号： C12Q1/44 ,  G01N21/64 ,  A61K31/35 ,  A61P11/06 ,  A61P37/08 ,  A61P35/00

CPC分类号： C12Q1/44 ,  A61K31/35

摘要： The present invention relates to a quantitative method for detecting yessotoxins in fishery products based on the activation the toxin produces on cellular phosphodiesterases and the therapeutic use of this activation. The cellular target of yessotoxin (YTX) and its analogs is the activation of phosphodiesterases (PDEs). The PDEs-YTX bond produces a measurable signal. The bond can be quantified by means of an affinity biosensor or by fluorescence. The biosensor detects biomolecular interactions and allows determining the presence of YTX due to its interaction with PDEs. The variations in the degradation rate of the fluorescent derivative anthraniloyl-cAMP are determined by means of plate fluorescence. The rate at which the PDEs degrade this molecule increases in the presence of YTX. YTX inhibits immunological activation of mastocytes in rats and induces a cytotoxic effect in human hepatocarcinoma cells, which implies two therapeutic uses of YTXs as an antiallergic and antitumor compound.

摘要翻译： 本发明涉及一种用于检测基于所述毒素对细胞磷酸二酯酶和治疗用途本活化的产生[活化在渔业产品yessotoxins定量的方法。 Yessotoxin（YTX）和它的类似物的细胞靶标是磷酸二酯酶（PDE）的活化。 偏微分方程-YTX键产生一个可测量的信号。 粘结可以通过亲和生物传感器的或通过荧光来定量。 生物传感器检测生物分子相互作用和允许开采确定性YTX的存在，因为它与偏微分方程的相互作用。 在荧光衍生物茴酰cAMP的降解速率的变化被确定由板荧光手段开采。 在其中的偏微分方程降低这种分子的速度在YTX的存在增加。 YTX抑制大鼠肥大细胞的免疫活化和诱导人肝癌细胞，这暗示YTXs两种治疗用途作为抗过敏药和抗肿瘤化合物的细胞毒性作用。