发明公开
- 专利标题： METHOD FOR THE QUANTIFICATION OF METHYLATED DNA
- 专利标题（中）： 方法定量甲基化的DNA
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申请号： EP05737974.5申请日： 2005-04-06
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公开(公告)号： EP1733054A2公开(公告)日： 2006-12-20
- 发明人: GÜTIG, David , HABIGHORST, Dirk , KLUTH, Antje , SCHMITT, Armin , SCHUSTER, Matthias , SCHWOPE, Ina
- 申请人： Epigenomics AG
- 申请人地址： Kleine Präsidentenstrasse 1 10178 Berlin DE
- 专利权人： Epigenomics AG
- 当前专利权人： Epigenomics AG
- 当前专利权人地址： Kleine Präsidentenstrasse 1 10178 Berlin DE
- 代理机构： Schubert, Klemens
- 优先权： EP04090133 20040406; EP04090213 20040528
- 国际公布： WO2005098035 20051020
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The method according to the invention concerns a method for the quantification of two different variations of a DNA sequence. Particularly, the invention relates to a quantification of methylated DNA. For this purpose, the DNA to be investigated is first converted so that cytosine is converted to uracil, while 5-methylcytosine remains unchanged. Then the converted DNA is amplified by means of a real-time PCR. However, probes are utilized, one of which is specific for the methylated status and one for the unmethylated status of the DNA. The degree of methylation of the investigated DNA can be calculated from the ratio of the signal intensities of the probes or from the Ct values. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer and other disorders associated with a change in the methylation status, as well as for the prediction of drug interactions.
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