发明公开
EP1746162A2 Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell
失效
编码双特异性蛋白和方法用于在动物的哺乳动物细胞生产生物活性的双特异性融合蛋白的表达载体
- 专利标题： Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell
- 专利标题（中）： 编码双特异性蛋白和方法用于在动物的哺乳动物细胞生产生物活性的双特异性融合蛋白的表达载体
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申请号： EP05077801.8申请日： 1994-01-31
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公开(公告)号： EP1746162A2公开(公告)日： 2007-01-24
- 发明人: Ledbetter, Jeffrey A. , Gilliland, Lisa K. , Hayden, Martha S. , Linsley, Peter S. , Bajorath, Jurgen, Rhein. Friedrich-Wilhelms Univ. , Fell, Perry H.
- 申请人： Bristol-Myers Squibb Company
- 申请人地址： 345 Park Avenue New York, N.Y. 10154 US
- 专利权人： Bristol-Myers Squibb Company
- 当前专利权人： Bristol-Myers Squibb Company
- 当前专利权人地址： 345 Park Avenue New York, N.Y. 10154 US
- 代理机构： Howard, Paul Nicholas
- 优先权： US13420 19930201; US121054 19930913
- 主分类号： C12N15/79
- IPC分类号： C12N15/79 ; C12N15/62 ; C07K16/46 ; C12N5/10 ; C07K16/28 ; C07K16/30
摘要：
The present invention provides an expression vector encoding monospecific or bispecific fusion protein.
In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen.
In another embodiment the expression vector encodes a. bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen.
The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell. This method comprises: (a) transfecting the mammalian cell with the recombinant expression vector of the invention; (b) culturing the mammalian cell so transfected in step (a); and (c) recovering the biologically active bispecific fusion protein so as produced by the cultured mammalian cell.
In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen.
In another embodiment the expression vector encodes a. bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen.
The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell. This method comprises: (a) transfecting the mammalian cell with the recombinant expression vector of the invention; (b) culturing the mammalian cell so transfected in step (a); and (c) recovering the biologically active bispecific fusion protein so as produced by the cultured mammalian cell.
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