A chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein, and a method of using the same are provided. A chemical reaction cartridge is provided with a substrate (1), and an elastic member 2 overlaid on the substrate (1). A recess of a predetermined shape, depressed toward the top surface of the elastic member (2) is formed in the back surface of the elastic member (2). The recess creates space between the substrate (1), and the elastic member (2), thereby making up a well (21), for holding the main ingredient of the epoxy adhesive, a flow path (22) communicating with the well (21), a well (23) for holding a curing agent for the epoxy adhesive, a flow path (24) communicating with the well (23), a mixing part (25) for mixing the main ingredient with the curing agent, and a discharge path (26) for discharging a mixture. While the chemical reaction cartridge is not in use as yet, the flow paths (22, 24), and the discharge path (26) are closed, so that the wells (21, 23) are, in effect, in a hermetically sealed condition. The mixing part (25) is made up of a plurality of flow paths (25a) intersecting each other. The respective starting ends of the flow paths (25a) are each linked with the flow path (22), or the flow path (24), and the respective terminals of the flow paths (25a) are linked with the discharge path (26). The terminal of the discharge path (26) communicates with the outside via an end face of the cartridge.