公开(公告)号：EP1769849A1

公开(公告)日：2007-04-04

申请号：EP06018888.5

申请日：2006-09-08

申请人： Yokogawa Electric Corporation

发明人： Tanaami, Takeo ,  Aoki, Hidetoshi ,  Satou, Saya ,  Mogi, Takeyuki

IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F5/06 ,  B01J19/00

CPC分类号： B01L3/502707 ,  B01F5/0641 ,  B01F5/0645 ,  B01F5/0693 ,  B01F5/0696 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/025 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/0086 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0481

摘要： A chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein, and a method of using the same are provided. A chemical reaction cartridge is provided with a substrate (1), and an elastic member 2 overlaid on the substrate (1). A recess of a predetermined shape, depressed toward the top surface of the elastic member (2) is formed in the back surface of the elastic member (2). The recess creates space between the substrate (1), and the elastic member (2), thereby making up a well (21), for holding the main ingredient of the epoxy adhesive, a flow path (22) communicating with the well (21), a well (23) for holding a curing agent for the epoxy adhesive, a flow path (24) communicating with the well (23), a mixing part (25) for mixing the main ingredient with the curing agent, and a discharge path (26) for discharging a mixture. While the chemical reaction cartridge is not in use as yet, the flow paths (22, 24), and the discharge path (26) are closed, so that the wells (21, 23) are, in effect, in a hermetically sealed condition. The mixing part (25) is made up of a plurality of flow paths (25a) intersecting each other. The respective starting ends of the flow paths (25a) are each linked with the flow path (22), or the flow path (24), and the respective terminals of the flow paths (25a) are linked with the discharge path (26). The terminal of the discharge path (26) communicates with the outside via an end face of the cartridge.

摘要翻译： 能够使化学反应稳定且安全地进行的化学反应筒及其使用方法。 化学反应盒设置有基板（1）和覆盖在基板（1）上的弹性部件2。 在弹性部件（2）的后表面形成有朝向弹性部件（2）的上表面凹陷的规定形状的凹部。 所述凹部在所述基板（1）和所述弹性部件（2）之间形成空间，从而构成用于保持所述环氧粘合剂的主要成分的孔（21），与所述孔（21）连通的流路（22） ），用于保持环氧树脂粘合剂的固化剂的井（23），与井（23）连通的流路（24），用于混合主成分与固化剂的混合部（25） 路径（26），用于排出混合物。 当化学反应盒尚未使用时，流路（22,24）和排出路径（26）关闭，使得井（21,23）实际上处于气密密封状态 。 混合部（25）由相互交叉的多个流路（25a）构成。 流路（25a）的各自的起始端各自与流路（22）或流路（24）连接，流路（25a）的各端子与排出路径（26）连结， 。 排出路径（26）的端子经由盒的端面与外部连通。

公开(公告)号：EP2282195A3

公开(公告)日：2011-09-28

申请号：EP10170178.7

申请日：2010-07-20

申请人： Yokogawa Electric Corporation

发明人： Tanaami, Takeo ,  Aoki, Hidetoshi ,  Sugiyama, Yumiko ,  Shimoda, Souichirou ,  Araragi, Muneki

IPC分类号： G01N21/64

CPC分类号： G01N21/6452 ,  G01N21/6428

摘要： An amount of fluorescent light (5) generated from the specimen (4) is quantitatively measured as "a light-amount value per unit area traceable to the national standard" instead of in terms of a ratio (a ratio value) as in the past. A method for measuring a fluorescent light amount, whereby a specimen (4) is irradiated with excitation light (2), and fluorescent light (5) generated from the specimen (4) is measured by a light-receiving element via a receiving optical system (6), said method comprising the steps of irradiating the specimen (4) with excitation light (2) having a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen (4) beforehand, causing the light-receiving element to measure the fluorescent light (5) generated from the specimen (4) via the receiving optical system (6), and computing a fluorescent light-amount as measured by the light-receiving element via the receiving optical system (6) on the basis of an excitation light-amount per the specified unit area, optical coefficients of the receiving optical system (6), and the acceptance coefficient of the light-receiving element, thereby working out a light amount value per the unit area traceable to the national standard.

公开(公告)号：EP1792654A2

公开(公告)日：2007-06-06

申请号：EP06019338.0

申请日：2006-09-15

申请人： Yokogawa Electric Corporation

发明人： Tanaami, Takeo ,  Aoki, Hidetoshi

IPC分类号： B01L3/00 ,  G01N33/543

CPC分类号： G01N33/54326 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54386

摘要： A cartridge capable of making effective use of a sample is provided. A blood sample is injected into a well (21) via an injection path (41) by use of a syringe, and so forth. A roller (6), in a state as kept pressed into contact with the cartridge, is rotated rightward, whereupon an elastic member (2) undergoes elastic deformation to cause the blood sample held in the well (21), and a liquid solvent held in a well (22) to reach a well (23), and the blood sample is thereby mixed with the liquid solvent. Since the liquid solvent contains surfactant, blood cells are destroyed in the well (23). The liquid solvent reaches a well (26) via a flow path (45). At this point in time, magnetic particles held in the well (25), and a cleaning liquid held in the well (24) are merged with the mixed liquid in the well (26). A magnet (7) is shifted from a position corresponding to the well (26) to a position corresponding to the well (31) along the flow path (46), thereby separating and transferring DNA caught by the magnetic particles to the well (31). Residue inside the well (26) after removal of DNA is transferred to a well (54) by the roller. The separated biopolymers are analyzed in the cartridge.

摘要翻译： 提供能够有效利用样品的盒。 血液样品通过注射器通过注射通道（41）注入孔（21）中，等等。 在保持与药筒接触的状态下的辊（6）向右旋转，于是弹性部件（2）经受弹性变形，使得保持在井（21）中的血液样本保持 在井（22）中以到达井（23），并且由此将血液样品与液体溶剂混合。 由于液体溶剂含有表面活性剂，所以在孔中破坏了血细胞（23）。 液体溶剂经由流路（45）到达井（26）。 此时，保持在井（25）内的磁性颗粒和保持在井（24）中的清洗液体与井26中的混合液体合并。 磁体（7）从对应于阱（26）的位置沿着流路（46）移动到对应于阱（31）的位置，从而将由磁性颗粒捕获的DNA分离并转移到井（31 ）。 去除DNA后，孔（26）内的残留物通过辊转移到孔（54）中。 在筒中分析分离的生物聚合物。

公开(公告)号：EP2282195A2

公开(公告)日：2011-02-09

申请号：EP10170178.7

申请日：2010-07-20

申请人： Yokogawa Electric Corporation

发明人： Tanaami, Takeo ,  Aoki, Hidetoshi ,  Sugiyama, Yumiko ,  Shimoda, Souichirou ,  Araragi, Muneki

IPC分类号： G01N21/64

CPC分类号： G01N21/6452 ,  G01N21/6428

摘要： An amount of fluorescent light (5) generated from the specimen (4) is quantitatively measured as "a light-amount value per unit area traceable to the national standard" instead of in terms of a ratio (a ratio value) as in the past. A method for measuring a fluorescent light amount, whereby a specimen (4) is irradiated with excitation light (2), and fluorescent light (5) generated from the specimen (4) is measured by a light-receiving element via a receiving optical system (6), said method comprising the steps of irradiating the specimen (4) with excitation light (2) having a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen (4) beforehand, causing the light-receiving element to measure the fluorescent light (5) generated from the specimen (4) via the receiving optical system (6), and computing a fluorescent light-amount as measured by the light-receiving element via the receiving optical system (6) on the basis of an excitation light-amount per the specified unit area, optical coefficients of the receiving optical system (6), and the acceptance coefficient of the light-receiving element, thereby working out a light amount value per the unit area traceable to the national standard.

摘要翻译： （5）从所述样品（4）的定量测​​量为“每单位面积的光量值可溯源至国家标准”，而不是在一个比计（比例值）作为在过去产生的荧光的光量 ， 一种用于测量荧光的光量，由此，试样（4）照射从试样产生的激发光（2），和荧光灯（5）方法（4）是由一个受光元件经由受光光学系统测量 （6）所述的方法包括用（2），其具有指定的每单位面积的可溯源至国家标准的光量值的激励光照射在试样（4）的步骤，和（4）预先指定的试样的表面上 ，使光接收元件来测量荧光的（5）经由所述受光光学系统（6），并计算荧光光量从样品（4）产生经由受光光学由光接收元件所测量 系统（6）每指定单位面积的激励光量的基础上，所述受光光学系统（6），以及光接收元件的受系数的光学系数，从而制定出每一个光量值 UNI T区可溯源至国家标准。

公开(公告)号：EP1914001A1

公开(公告)日：2008-04-23

申请号：EP08001244.6

申请日：2006-09-08

申请人： Yokogawa Electric Corporation

发明人： Tanaami, Takeo ,  Aoki, Hidetoshi ,  Satou, Saya ,  Mogi, Takeyuki

IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F5/06 ,  B01J19/00

CPC分类号： B01L3/502707 ,  B01F5/0641 ,  B01F5/0645 ,  B01F5/0693 ,  B01F5/0696 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/025 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/0086 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0481

摘要： The invention relates to a chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein upon transferring substances contained therein to thereby apply a chemical reaction, and a method of using the same. Said cartridge comprises a plurality of holding parts (60,61) for holding the substances therein, respectively, and a discharge part for discharging a mixture of the substances to the outside of the cartridge, in accordance with the deformation of the cartridge, wherein the substances each are a fluid and fine particles (62), and the mixture of the fluid and the fine particles is discharged from the discharge part.

摘要翻译： 本发明涉及使化学反应能够在其中转移含有物质的化学反应稳定且安全地进行化学反应，从而进行化学反应及其使用方法。 所述筒体分别包括用于将物质保持在其中的多个保持部分（60,61）和用于将物质的混合物排出到盒的外部的排出部分，根据筒的变形，其中 物质各自是流体和细颗粒（62），并且流体和细颗粒的混合物从排出部分排出。

公开(公告)号：EP1792654A3

公开(公告)日：2007-10-03

申请号：EP06019338.0

申请日：2006-09-15

申请人： Yokogawa Electric Corporation

发明人： Tanaami, Takeo ,  Aoki, Hidetoshi

IPC分类号： B01L3/00 ,  G01N33/543

CPC分类号： G01N33/54326 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54386

摘要： A cartridge capable of making effective use of a sample is provided. A blood sample is injected into a well (21) via an injection path (41) by use of a syringe, and so forth. A roller (6), in a state as kept pressed into contact with the cartridge, is rotated rightward, whereupon an elastic member (2) undergoes elastic deformation to cause the blood sample held in the well (21), and a liquid solvent held in a well (22) to reach a well (23), and the blood sample is thereby mixed with the liquid solvent. Since the liquid solvent contains surfactant, blood cells are destroyed in the well (23). The liquid solvent reaches a well (26) via a flow path (45). At this point in time, magnetic particles held in the well (25), and a cleaning liquid held in the well (24) are merged with the mixed liquid in the well (26). A magnet (7) is shifted from a position corresponding to the well (26) to a position corresponding to the well (31) along the flow path (46), thereby separating and transferring DNA caught by the magnetic particles to the well (31). Residue inside the well (26) after removal of DNA is transferred to a well (54) by the roller. The separated biopolymers are analyzed in the cartridge.