The invention relates to a method for determining binding parameters between at least one type of substance and at least one binding partner by using at least one luminescent probe and an appropriate kit. For carrying out the inventive method, a solid body which is at least partially loaded with at least one binding partner and which is incubated with the testable substance is used. After separation of the solid body and a non-bound substance, the concentration of the testable substance is determined by measuring the modulation or weakening of luminescence signals of at least one probe by the non-bound substance in a supernatant.