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公开(公告)号：EP1922538A1
公开(公告)日：2008-05-21
申请号：EP06777169.1
申请日：2006-09-06
IPC分类号： G01N15/08
CPC分类号： G01N15/0826 , G01N2015/0846 , G01N2015/0873
摘要： The invention relates to a method for measuring the permeation of at least one substance contained in a solvent through a permeation barrier and/or determining parameters influencing said permeation, especially the permeability of the barrier for the at least one substance, with the aid of at least one luminescent probe. According to the inventive method, the at least one substance is provided on one side of the permeation barrier, the permeation barrier is incubated with the at least one substance contained in the solvent, and luminescence signals of the probe, which are modulated in accordance with the respective concentration of the substance, are measured on at least one side of the permeation barrier, the excitation spectrum and/or the emission spectrum of the at least one probe overlapping with the absorption spectrum of the at least one substance. Also disclosed is a kit to be used in said method.
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公开(公告)号：EP1325334B1
公开(公告)日：2011-11-30
申请号：EP01980432.7
申请日：2001-09-25
发明人： NÖLLER, Joachim , SCHMITT, Johannes
IPC分类号： G01N33/543
CPC分类号： G01N33/54353 , G01N33/5436 , G01N33/54393
摘要： The invention relates to a method for immobilising lipid layers on the surfaces of solid bodies, according to which the surfaces of said solid bodies are modified in such a way that the characteristics of the lipid layers deposited thereon, in particular with regard to their diffusive capacity, largely resemble those of non-immobilised lipid layers. According to the method, the surface of the solid body is first modified by means of molecules, in such a way that a substantially hydrophilic surface is formed. In a second step, lipid layers, in particular, lipid bilayers are deposited on said modified surface.
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公开(公告)号：EP1381863A1
公开(公告)日：2004-01-21
申请号：EP02732658.6
申请日：2002-04-24
发明人： SCHMITT, Johannes , NÖLLER, Joachim
IPC分类号： G01N33/543
CPC分类号： G01N33/5432 , Y10T428/2991
摘要： The invention relates to a system, which can be used for measuring membrane permeation in substances. Said system comprises, essentially, porous particles with an inner surface formed within the pores, and another outer surface. Essentially, only the outer surface is fully covered by a lipid layer, said lipid layer extending over the openings of the pores on the outer surface. Preferably, an intermediate layer is arranged between the outer surface and the lipid layer. Said intermediate layer is embodied, more particularly, in the form a polymer network.
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公开(公告)号：EP1325334A2
公开(公告)日：2003-07-09
申请号：EP01980432.7
申请日：2001-09-25
发明人： NÖLLER, Joachim , SCHMITT, Johannes
IPC分类号： G01N33/543
CPC分类号： G01N33/54353 , G01N33/5436 , G01N33/54393
摘要： The invention relates to a method for immobilising lipid layers on the surfaces of solid bodies, according to which the surfaces of said solid bodies are modified in such a way that the characteristics of the lipid layers deposited thereon, in particular with regard to their diffusive capacity, largely resemble those of non-immobilised lipid layers. According to the method, the surface of the solid body is first modified by means of molecules, in such a way that a substantially hydrophilic surface is formed. In a second step, lipid layers, in particular, lipid bilayers are deposited on said modified surface.
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公开(公告)号：EP1381863B1
公开(公告)日：2006-11-15
申请号：EP02732658.6
申请日：2002-04-24
发明人： SCHMITT, Johannes , NÖLLER, Joachim
IPC分类号： G01N33/543
CPC分类号： G01N33/5432 , Y10T428/2991
摘要： The invention relates to a system, which can be used for measuring membrane permeation in substances. Said system comprises, essentially, porous particles with an inner surface formed within the pores, and another outer surface. Essentially, only the outer surface is fully covered by a lipid layer, said lipid layer extending over the openings of the pores on the outer surface. Preferably, an intermediate layer is arranged between the outer surface and the lipid layer. Said intermediate layer is embodied, more particularly, in the form a polymer network.
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公开(公告)号：EP1407268A2
公开(公告)日：2004-04-14
申请号：EP02758194.1
申请日：2002-05-14
发明人： SCHMITT, Johannes , NÖLLER, Joachim
IPC分类号： G01N33/543
CPC分类号： G01N33/553 , G01N33/54326 , G01N33/54386 , G01N33/552 , G01N2446/80
摘要： The invention relates to a method suitable for the production of immobilized double lipid layers, comprising an inner and an outer lipid layer. The inner and outer lipid layer of said double lipid layers have a defined orientation on the surface of a solid body and are immobilized on the surface of said solid body in a substantially stable and dynamic manner. In order to carry out the inventive method, the surface of a solid body is initially modified in such a way that a surface is formed which preferably interacts with only one of the two lipid layers. In a second step, a double lipid layer is deposited on said modified surface. Preferably, other components, especially protein, are inserted into the double lipid layer.
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公开(公告)号：EP1782045A1
公开(公告)日：2007-05-09
申请号：EP05769619.7
申请日：2005-07-29
IPC分类号： G01N21/82 , G01N33/543 , G01N33/542 , G01N33/58 , G01N21/64
CPC分类号： G01N33/54306 , G01N21/6428 , G01N33/582
摘要： The invention relates to a method for determining binding parameters between at least one type of substance and at least one binding partner by using at least one luminescent probe and an appropriate kit. For carrying out the inventive method, a solid body which is at least partially loaded with at least one binding partner and which is incubated with the testable substance is used. After separation of the solid body and a non-bound substance, the concentration of the testable substance is determined by measuring the modulation or weakening of luminescence signals of at least one probe by the non-bound substance in a supernatant.
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