The invention relates to a chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein upon transferring substances contained therein to thereby apply a chemical reaction, and a method of using the same. Said cartridge comprises a plurality of holding parts (60,61) for holding the substances therein, respectively, and a discharge part for discharging a mixture of the substances to the outside of the cartridge, in accordance with the deformation of the cartridge, wherein the substances each are a fluid and fine particles (62), and the mixture of the fluid and the fine particles is discharged from the discharge part.