发明公开
EP1930730A2 Systems and methods to perform assays for detecting or quantifying analytes
审中-公开
系统和用于分析物的检测或定量进行测定的方法
- 专利标题: Systems and methods to perform assays for detecting or quantifying analytes
- 专利标题(中): 系统和用于分析物的检测或定量进行测定的方法
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申请号: EP08003069.5申请日: 2006-03-10
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公开(公告)号: EP1930730A2公开(公告)日: 2008-06-11
- 发明人: Carrick, James M. , Davis, Christopher Brandon , Heinz, Robert E. , Knight, Byron J. , Lair, Gary D. , Li, Florence F. , Li, Haito , Light, James P. , Macioszek, Jerzy A. , Nguyen, Thanh N. , Scalese, Robert F.
- 申请人: GEN-PROBE INCORPORATED
- 申请人地址: 10210 Genetic Center Drive San Diego, CA 92121 US
- 专利权人: GEN-PROBE INCORPORATED
- 当前专利权人: GEN-PROBE INCORPORATED
- 当前专利权人地址: 10210 Genetic Center Drive San Diego, CA 92121 US
- 代理机构: Lloyd, John Scott
- 优先权: US659874P 20050310
- 主分类号: G01N35/02
- IPC分类号: G01N35/02
摘要:
A system for processing the contents of multiple reaction receptacles to detennine the amount of a target nucleic acid present in one or more of the reaction receptacles or to ascertain the presence or absence of a target nucleic acid in one or more of the reaction receptacles, said system comprising: (A) at least one temperature-controlled incubator including an incubation chamber for receiving reaction receptacles therein and for exposing the reaction receptacles within said incubation chamber to controlled temperature conditions; (B) a nucleic acid quantification system constructed and arranged to determine the amount of a target nucleic acid within the reaction receptacles by: (1) periodically measuring the amount of a signal emitted by the contents of each reaction receptacle; and (2) quantifying the amount of the target nucleic acid present in each reaction receptacle based upon the periodic measurements; and (C) a nucleic acid detection system constructed and arranged to ascertain the presence of the target nucleic acid within the reaction receptacle by: (1) detecting a signal emitted by the contents each reaction receptacle; and (2) ascertaining the presence or absence of the target nucleic acid in the reaction receptacle based upon whether or not a signal above a threshold level is detected.
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