公开(公告)号：EP1909108B1

公开(公告)日：2019-05-29

申请号：EP08001232.1

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

公开(公告)号：EP1930730A3

公开(公告)日：2014-05-28

申请号：EP08003069.5

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

摘要： A system for processing the contents of multiple reaction receptacles to detennine the amount of a target nucleic acid present in one or more of the reaction receptacles or to ascertain the presence or absence of a target nucleic acid in one or more of the reaction receptacles, said system comprising: (A) at least one temperature-controlled incubator including an incubation chamber for receiving reaction receptacles therein and for exposing the reaction receptacles within said incubation chamber to controlled temperature conditions; (B) a nucleic acid quantification system constructed and arranged to determine the amount of a target nucleic acid within the reaction receptacles by: (1) periodically measuring the amount of a signal emitted by the contents of each reaction receptacle; and (2) quantifying the amount of the target nucleic acid present in each reaction receptacle based upon the periodic measurements; and (C) a nucleic acid detection system constructed and arranged to ascertain the presence of the target nucleic acid within the reaction receptacle by: (1) detecting a signal emitted by the contents each reaction receptacle; and (2) ascertaining the presence or absence of the target nucleic acid in the reaction receptacle based upon whether or not a signal above a threshold level is detected.

公开(公告)号：EP1930730B1

公开(公告)日：2019-08-14

申请号：EP08003069.5

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

公开(公告)号：EP1921454B1

公开(公告)日：2015-08-12

申请号：EP08003851.6

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

公开(公告)号：EP2322940A1

公开(公告)日：2011-05-18

申请号：EP10010799.4

申请日：2006-03-10

申请人： Gen-Probe Incorporated

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Bryon J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02 ,  C12Q1/68

CPC分类号： G01N21/6428 ,  B01L7/52 ,  C12Q1/6813 ,  C12Q1/6851 ,  G01N21/6454 ,  G01N21/76 ,  G01N35/00693 ,  G01N35/0092 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/026 ,  G01N35/028 ,  G01N35/04 ,  G01N35/1002 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2035/00346 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/0097 ,  G01N2035/0441 ,  G01N2035/0443 ,  G01N2035/0444 ,  G01N2035/0455 ,  G01N2035/0491 ,  G01N2035/103 ,  G01N2201/0415 ,  Y10S435/808 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/25 ,  C12Q2537/165

摘要： A computer-implemented method for determining the amount of an analyte of interest in a sample, the method comprising (a) forming amplification products from an analyte of interest, (b) collecting signal data with a signal measuring device concurrently with the formation of amplification products, the collected signal data comprising periodic measurements of the level of a signal emitted by the sample, wherein the level of signal emitted by the sample corresponds to the amount of amplification products formed by the sample, (c) storing the collected signal data in computer-readable media; and (d) using a computer programmed with an algorithm, which, when executed by the computer, is configured to cause the computer to access the data stored in step (c) and to adjust the data collected in step (b) to remove background contributions to the measured levels associated with sources other than the presence of the analyte of interest, normalize the data adjusted in step (d)(1) by dividing the adjusted data by the maximum level measured, adjusted to remove background contributions, approximate a curve through a portion of the adjusted, normalized data between a predetermined low bound stored on computer-readable media and a predetermined high bound stored on computer-readable media, determine an emergence time at which the curve approximated in step (d)(3) crosses a predetermined threshold data level and determine the amount of the analyte of interest by comparing the emergence time determined in step (d)(4) with threshold times determined for known amounts of the analyte of interest and stored on computer-readable media.

摘要翻译： 确定性采矿感兴趣的样品中的分析物的量的计算机实现的方法，所述方法包括（a）形成的扩增产物从与感兴趣的分析物，（b）收集信号数据用的信号与放大的形成测量装置兼 产品，所收集的信号数据包括：由所述样品发射的信号的电平的周期性测量，worin由样品发射的信号的电平对应于扩增产物通过样品，（c）中形成的量在存储所收集的信号数据 计算机可读介质; 以及使用与在算法，其编程的计算机（d）中，当由计算机执行时，被配置为使访问存储在步骤（c）的数据，并调整在步骤（b）以去除背景收集的数据的计算机 到具有比所关注的分析物的存在其他源相关联的测量水平的贡献，由测得的最大电平，调节到去除背景的贡献将经调整的数据归一化在步骤（d）调整的数据（1），近似的曲线 通过结合存储在计算机可读介质中的预定的低和结合的预定的高存储在计算机可读介质上，在在哪个曲线，在步骤（d）中的逼近出苗时间确定性矿之间的调整，归一化的数据的一个部分（3）的杂交 预定阈值水平的数据和确定的矿通过比较在步骤（d）开采的出现时间的确定性的感兴趣的分析物的量（4）与阈值时间确定开采的中的分析物的已知量的 terest和存储在计算机可读介质上。

公开(公告)号：EP1909108A3

公开(公告)日：2014-05-28

申请号：EP08001232.1

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

摘要： A method for processing the contents of multiple reaction receptacles to quantitatively determine the amount of a target nucleic acid present in each of said reaction receptacles, said method comprising the automated steps of a) successively providing to a heater a plurality of reaction receptacles, each reaction receptacle being provided with reagents sufficient to amplify and detect the presence of a target sequence, or its complement, contained within a target nucleic acid under the temperature conditions of said heater; b) periodically measuring the amount of a signal associated with the presence of an amplification product containing said target sequence or its complement in each reaction receptacle provided to said heater in accordance with step a); and c) quantifying the amount of said target nucleic acid present in each reaction receptacle based upon said measurements obtained in step b), wherein a portion of said plurality of reaction receptacles are being successively provided to said heater in accordance with step a) as the contents of a portion of said plurality of reaction receptacles are being processed in accordance with steps b)-c).

摘要翻译： 一种用于处理多个反应容器的内容物定量的确定性矿靶核酸存在于每个所述反应容器的酸的量，所述方法包括的自动化步骤）依次提供给一个加热器的反应容器的复数，每个反应方法 容器设置有足够的试剂，以扩增和检测靶序列的存在，或其互补物，包含在所述加热器的温度条件下与靶核酸内; b）中周期性地测量与扩增产物含有所述靶序列或在其提供给所述加热器在雅舞蹈与步骤a每个反应容器）补体的存在相关联的信号的量; 和c）定量所述的量靶核酸存在于基于在步骤B中获得所述测量每个反应容器酸），worin所述多个的一部分的反应容器被连续地提供给所述加热器在雅舞蹈与步骤a）中作为 反应容器的所述多个的一部分的内容在雅舞蹈正在处理与步骤b）中-c）。

公开(公告)号：EP1921454A3

公开(公告)日：2008-09-17

申请号：EP08003851.6

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

摘要： The present invention relates to a system for measuring a signal emitted by the contents of each of a plurality of reaction receptacles comprising: a moving reaction receptacle carrier including a plurality of reaction receptacle stations, each of said reaction receptacle stations carrying two or more reaction receptacles; a signal measuring device constructed and arranged to measure the amount of a signal emitted by the contents of a reaction receptacle placed in operative proximity to said signal measuring device, said signal measuring device being positioned with respect to said reaction receptacle carrier such that reaction receptacles carried on said reaction receptacle carrier are successively moved into operative proximity to said signal measuring device as said reaction receptacle carrier moves the reaction receptacles; and a signal measuring device transport mechanism constructed and arranged to move said signal measuring device with respect to said reaction receptacle carrier, wherein said reaction receptacle carrier is controlled so as to successively present each said reaction receptacle station in a signal measuring position in which one of the reaction receptacles carried in the reaction receptacle station is in operative proximity to said signal measuring device and said signal measuring device transport mechanism is controlled so as to move said signal measuring device with respect to said reaction receptacle carrier to successively place said signal measuring device in operative proximity to each of the reaction receptacles carried in the reaction receptacle station moved into the signal measuring position.

公开(公告)号：EP1930730A2

公开(公告)日：2008-06-11

申请号：EP08003069.5

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

摘要： A system for processing the contents of multiple reaction receptacles to detennine the amount of a target nucleic acid present in one or more of the reaction receptacles or to ascertain the presence or absence of a target nucleic acid in one or more of the reaction receptacles, said system comprising: (A) at least one temperature-controlled incubator including an incubation chamber for receiving reaction receptacles therein and for exposing the reaction receptacles within said incubation chamber to controlled temperature conditions; (B) a nucleic acid quantification system constructed and arranged to determine the amount of a target nucleic acid within the reaction receptacles by: (1) periodically measuring the amount of a signal emitted by the contents of each reaction receptacle; and (2) quantifying the amount of the target nucleic acid present in each reaction receptacle based upon the periodic measurements; and (C) a nucleic acid detection system constructed and arranged to ascertain the presence of the target nucleic acid within the reaction receptacle by: (1) detecting a signal emitted by the contents each reaction receptacle; and (2) ascertaining the presence or absence of the target nucleic acid in the reaction receptacle based upon whether or not a signal above a threshold level is detected.

摘要翻译： 一种用于处理多个反应容器的内容物的端部9的靶核酸中存在的一个或多个反应容器的或，以确定在一个或多个反应容器，称靶核酸的存在或不存在的量系统 系统，包括：（A）至少一个温度可控的培养箱上孵育室包括用于接收反应容器于其中并用于暴露所述孵育室中的反应容器，以受控的温度条件; （B）构建和布置成确定性矿反应容器内的靶核酸的量的核酸定量系统：（1）周期性地测量由每个反应容器的内容物发出的信号的量; 和（2）定量靶核酸存在于基于所述周期性测量每个反应容器中的酸的量; 和（C）由构造和布置为确定反应容器内的目标核酸的存在的核酸检测系统：（1）检测由所述内容的每个反应容器发射的信号; 和（2）确定所述靶核酸的在基于是否检测到高于阈值电平的信号在反应容器中的存在或不存在。

公开(公告)号：EP1921454A2

公开(公告)日：2008-05-14

申请号：EP08003851.6

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

摘要： The present invention relates to a system for measuring a signal emitted by the contents of each of a plurality of reaction receptacles comprising: a moving reaction receptacle carrier including a plurality of reaction receptacle stations, each of said reaction receptacle stations carrying two or more reaction receptacles; a signal measuring device constructed and arranged to measure the amount of a signal emitted by the contents of a reaction receptacle placed in operative proximity to said signal measuring device, said signal measuring device being positioned with respect to said reaction receptacle carrier such that reaction receptacles carried on said reaction receptacle carrier are successively moved into operative proximity to said signal measuring device as said reaction receptacle carrier moves the reaction receptacles; and a signal measuring device transport mechanism constructed and arranged to move said signal measuring device with respect to said reaction receptacle carrier, wherein said reaction receptacle carrier is controlled so as to successively present each said reaction receptacle station in a signal measuring position in which one of the reaction receptacles carried in the reaction receptacle station is in operative proximity to said signal measuring device and said signal measuring device transport mechanism is controlled so as to move said signal measuring device with respect to said reaction receptacle carrier to successively place said signal measuring device in operative proximity to each of the reaction receptacles carried in the reaction receptacle station moved into the signal measuring position.

摘要翻译： 本发明涉及一种系统，用于测量由每个反应容器包括多个的内容物发射的信号：移动反应容器载体包含反应容器台的多个，每个所述反应容器站的带有两个或多个反应容器 ; 信号测量装置被构造和布置成测量通过在操作接近所述信号测量装置放置在反应容器的内容物发出的信号的量，所述信号测量装置被定位相对于所述反应容器载体搜索没有反应容器携带 所述载体上的反应容器依次移动到可操作接近所述信号测量装置，所述反应容器载体移动该反应容器; 和信号测量装置传送机构构造和布置成移动所述信号相对于所述反应容器载体测量装置，worin所述反应容器载体被控制，从而连续呈现每个所述反应容器站中的信号的测量位置，其中之一 在反应容器站中的反应容器处于操作接近所述信号测量装置和所述信号测量装置传送机构被控制，以使所述信号测量装置相对于所述反应容器载体以连续地放置所述信号测量装置中 操作邻近于每个在反应容器站中的反应容器的移动到信号的位置测量。

公开(公告)号：EP1909108A2

公开(公告)日：2008-04-09

申请号：EP08001232.1

申请日：2006-03-10

申请人： GEN-PROBE INCORPORATED

发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Byron J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

IPC分类号： G01N35/02

CPC分类号： G01N35/025 ,  G01N35/04 ,  G01N2035/00346

摘要： A method for processing the contents of multiple reaction receptacles to quantitatively determine the amount of a target nucleic acid present in each of said reaction receptacles, said method comprising the automated steps of a) successively providing to a heater a plurality of reaction receptacles, each reaction receptacle being provided with reagents sufficient to amplify and detect the presence of a target sequence, or its complement, contained within a target nucleic acid under the temperature conditions of said heater; b) periodically measuring the amount of a signal associated with the presence of an amplification product containing said target sequence or its complement in each reaction receptacle provided to said heater in accordance with step a); and c) quantifying the amount of said target nucleic acid present in each reaction receptacle based upon said measurements obtained in step b), wherein a portion of said plurality of reaction receptacles are being successively provided to said heater in accordance with step a) as the contents of a portion of said plurality of reaction receptacles are being processed in accordance with steps b)-c).

摘要翻译： 一种用于处理多个反应容器的内容物以定量确定存在于每个所述反应容器中的目标核酸的量的方法，所述方法包括以下自动化步骤：a）向加热器连续提供多个反应容器，每个反应 容器提供足以放大并检测在所述加热器的温度条件下靶核酸内所含的靶序列或其互补物的存在的试剂; b）根据步骤a）定期测量与提供给所述加热器的每个反应容器中含有所述靶序列或其互补物的扩增产物的存在相关的信号的量; 和c）基于在步骤b）中获得的所述测量结果，定量存在于每个反应容器中的所述目标核酸的量，其中根据步骤a）将所述多个反应容器的一部分依次提供给所述加热器， 根据步骤b）-c）处理所述多个反应容器的一部分的内容物。