发明公开
- 专利标题： HIGH PRODUCTION SYSTEM FOR INFECTIOUS HEPATITIS C VIRUS PARTICLE
- 专利标题（中）： 高生产系统对感染丙型肝炎病毒颗粒
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申请号： EP06810936申请日： 2006-09-29
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公开(公告)号： EP1956087A4公开(公告)日： 2009-05-27
- 发明人: TANABE JUN-ICHI , SONE SABURO , WAKITA TAKAJI , ISHII KOJI , SUZUKI RYOSUKE , SUZUKI TETSURO , MIYAMURA TATSUO
- 申请人： JP NAT INST INFECTIOUS DISEASE , TOKYO METROPOLITAN ORG MED RES , TORAY INDUSTRIES
- 专利权人： JP NAT INST INFECTIOUS DISEASE,TOKYO METROPOLITAN ORG MED RES,TORAY INDUSTRIES
- 当前专利权人： JP NAT INST INFECTIOUS DISEASE,TOKYO METROPOLITAN ORG MED RES,TORAY INDUSTRIES
- 优先权： JP2005287646 2005-09-30
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N7/00
摘要：
The invention relates to a method for producing an infectious hepatitis C virus (HCV) particle, comprising the step of introducing an expression vector containing a DNA fragment including a DNA sequence encoding a 5’ noncoding region and a structural protein of HCV and an arbitrary nonstructural protein and a DNA sequence encoding a nonstructural protein and a 3’ noncoding region derived from HCV JFH1 strain downstream of the RNA polymerase I promoter and further containing a DNA fragment including the RNA polymerase I terminator downstream thereof into a cell which allows HCV proliferation.
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