公开(公告)号：EP1930416A4

公开(公告)日：2009-03-11

申请号：EP06810937

申请日：2006-09-29

申请人： JP NAT INST INFECTIOUS DISEASE ,  TOKYO METROPOLITAN ORG MED RES ,  TORAY INDUSTRIES

发明人： TANABE JUN-ICHI ,  SONE SABURO ,  WAKITA TAKAJI ,  ISHII KOJI ,  SUZUKI RYOSUKE ,  SUZUKI TETSURO ,  MIYAMURA TATSUO

IPC分类号： C12N7/00 ,  C12N7/04 ,  C12N15/09

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24223 ,  C12N2770/24243 ,  C12N2770/24252

摘要： The invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising introducing into (i) a cell which autonomously replicates an RNA replicon including a base sequence encoding a 5’ noncoding region, NS3 protein, NS4A protein, NS4B protein, NS5A protein and NS5B protein in the genomic RNA derived from a hepatitis C virus strain and a base sequence including a 3’ noncoding region, (ii) a vector expressing Core protein, E1 protein, E2 protein and p7 protein derived from the same or a different hepatitis C virus strain in (i), culturing the cell and collecting the produced virus-like particle and a recombinant hepatitis C virus particle produced by the method.

公开(公告)号：EP1956087A4

公开(公告)日：2009-05-27

申请号：EP06810936

申请日：2006-09-29

申请人： JP NAT INST INFECTIOUS DISEASE ,  TOKYO METROPOLITAN ORG MED RES ,  TORAY INDUSTRIES

发明人： TANABE JUN-ICHI ,  SONE SABURO ,  WAKITA TAKAJI ,  ISHII KOJI ,  SUZUKI RYOSUKE ,  SUZUKI TETSURO ,  MIYAMURA TATSUO

IPC分类号： C12N15/09 ,  C12N7/00

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24243 ,  C12N2770/24252

摘要： The invention relates to a method for producing an infectious hepatitis C virus (HCV) particle, comprising the step of introducing an expression vector containing a DNA fragment including a DNA sequence encoding a 5’ noncoding region and a structural protein of HCV and an arbitrary nonstructural protein and a DNA sequence encoding a nonstructural protein and a 3’ noncoding region derived from HCV JFH1 strain downstream of the RNA polymerase I promoter and further containing a DNA fragment including the RNA polymerase I terminator downstream thereof into a cell which allows HCV proliferation.

公开(公告)号：EP1457211A4

公开(公告)日：2006-06-14

申请号：EP01274763

申请日：2001-11-20

申请人： JAPAN SCIENCE & TECH AGENCY ,  JP NAT INST INFECTIOUS DISEASE

发明人： HONDA MITSUO ,  MATSUO KAZUHIRO ,  OHSU TAKEAKI ,  MIYAMURA TATSUO ,  MATSUURA YOSHIHARU ,  ISHII KOJI ,  KATO KENZO

IPC分类号： A61K39/00 ,  C07K14/16 ,  C12N7/01 ,  C12N7/08 ,  C12N15/49 ,  C12N15/863 ,  A61K39/285 ,  A61K39/21 ,  C12P21/00

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/24043 ,  C12N2740/16122

摘要： The invention provides a recombinant vaccinia virus strain DIs that possesses a polynucleotide encoding a foreign antigenic protein in the non-essential gene region of the chromosome DNA and expresses the antigenic protein; and provides a highly-safe, vaccinia virus vaccine containing the recombinant virus strain DIs as the active ingredient. The invention also provides a method of using the vaccinia virus strain DIs as a vector for protein expression.