发明公开
- 专利标题： REPAIR OF NUCLEIC ACIDS FOR IMPROVED AMPLIFICATION
- 专利标题（中）： NUCLEIC对改进功放维修
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申请号： EP07755155.4申请日： 2007-04-11
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公开(公告)号： EP2010678A2公开(公告)日： 2009-01-07
- 发明人: EVANS, Thomas, C. , SLATKO, Barton , CHEN, Lixin , VAISVILA, Romaldas , GUAN, Chudi , KUCERA, Rebecca
- 申请人： NEW ENGLAND BIOLABS, INC.
- 申请人地址： 240 County Road Ipswich, MA 01938 US
- 专利权人： NEW ENGLAND BIOLABS, INC.
- 当前专利权人： NEW ENGLAND BIOLABS, INC.
- 当前专利权人地址： 240 County Road Ipswich, MA 01938 US
- 代理机构： Froud, Clive
- 优先权： US791056P 20060411
- 国际公布： WO2007120627 20071025
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/o yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a DNA ligase and an effective amount of at least one endonuclease as well as a cofactor selected from NAD+ or ATP.
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