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    ROLLING CIRCLE REVERSE TRANSCRIPTION OF CIRCULAR RNA
    3.
    发明公开
    ROLLING CIRCLE REVERSE TRANSCRIPTION OF CIRCULAR RNA 审中-公开

    公开(公告)号：EP4137582A1

    公开(公告)日：2023-02-22

    申请号：EP22190872.6

    申请日：2022-08-17

    申请人： NEW ENGLAND BIOLABS, INC.

    发明人： GUAN, Shengxi ,  MAGUIRE, Sean ,  XU, Yan ,  UNLU, Irem

    IPC分类号： C12Q1/6844

    摘要： Compositions, methods and kits are provided that enable the detection, analysis and/or sequencing of small or large target RNA molecules whether synthetic, purified or within a biological fluid, or in cell lysate that may contain non-target RNA and other contaminating molecules without the need for depletion or purification steps that diminish what might already be low concentrations of the target molecule. The methods, compositions and kits rely on the use of a Group II Intron reverse transcriptase (Intron-RT) that have strand displacing properties and can generate concatemers in cDNA by rolling circle transcription of circRNAs that may be naturally circular or circularized in vitro from linear RNA.

    POLYNUCLEOTIDE ADAPTER DESIGN FOR REDUCED BIAS
    4.
    发明公开
    POLYNUCLEOTIDE ADAPTER DESIGN FOR REDUCED BIAS 审中-公开

    公开(公告)号：EP4119679A1

    公开(公告)日：2023-01-18

    申请号：EP22179442.3

    申请日：2020-04-24

    申请人： New England Biolabs, Inc.

    发明人： GUAN, Shengxi ,  MAGUIRE, Sean

    IPC分类号： C12Q1/6855 ,  C12N15/10

    摘要： Compositions and methods of use are provided that among other things, allow for efficient adapter ligation to small RNAs. Embodiments of the compositions include partially double stranded polynucleotides for use as 3' adapters that contain a cleavable linker positioned between a single-stranded region and a doublestranded region. Upon ligating the 3' adapters, the single-stranded region is released by cleaving the cleavable linker.

    PROTEINASES WITH IMPROVED PROPERTIES
    9.
    发明公开
    PROTEINASES WITH IMPROVED PROPERTIES 审中-公开

    公开(公告)号：EP4067486A1

    公开(公告)日：2022-10-05

    申请号：EP22164661.5

    申请日：2019-12-20

    申请人： New England Biolabs, Inc.

    发明人： CHEN, Minyong ,  SAMUELSON, James C. ,  XU, Ming-Oun ,  ZHANG, Aihua ,  HEIDER, Margaret ,  LIU, Pingfang

    IPC分类号： C12N9/50 ,  C12Q1/00

    摘要： Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in a hydrophobic core region of helix 3 corresponding to amino acids 230-237 of SEQ ID NO:1. The thermolabile proteinase is active at a temperature in the range of 4°C- 40°C and is inactivated by raising the temperature to above 50°C, where the proteinase is substantially inactive at 65°C.

    POLYNUCLEOTIDE ADAPTER DESIGN FOR REDUCED BIAS
    10.
    发明公开
    POLYNUCLEOTIDE ADAPTER DESIGN FOR REDUCED BIAS 审中-公开

    公开(公告)号：EP3730628A1

    公开(公告)日：2020-10-28

    申请号：EP20171272.6

    申请日：2020-04-24

    申请人： NEW ENGLAND BIOLABS, INC.

    发明人： GUAN, Shengxi ,  MAGUIRE, Sean

    IPC分类号： C12Q1/6855 ,  C12N15/10

    摘要： Compositions and methods of use are provided that among other things, allow for efficient adapter ligation to small RNAs. Embodiments of the compositions include partially double stranded polynucleotides for use as 3' adapters that contain a cleavable linker positioned between a single-stranded region and a doublestranded region. Upon ligating the 3' adapters, the single-stranded region is released by cleaving the cleavable linker.