The invention relates to a process for finding suitable parameters for the chromatographic purification of biomolecules. The process consists of equilibration, loading, washing and elution steps, wherein this sequence of steps is carried out in the partial batch process. The parameters determined in small, preferably a plurality, of parallel batches give information on the chromatography conditions under which a given biomolecule may be optimally purified by column chromatography, if appropriate even on a relatively large scale.