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公开(公告)号：EP2195431A1
公开(公告)日：2010-06-16
申请号：EP08803328.7
申请日：2008-08-28
IPC分类号： C12N15/13 , C12N15/85 , C07K16/00 , C07K14/505 , C12N9/72
CPC分类号： C07K16/00 , C07K16/40 , C07K2317/24 , C07K2317/52
摘要： The invention relates to methods for increasing the titer of a protein of interest in a cell as well as enhancing production and purification of optimized biomolecules, the component of which is, inter alia, the CH3 domain. In biomolecules, the C-terminal amino acid(s), e.g. the C-terminal lysine, frequently separate(s). The mostly incomplete processing of, for example, the heavy chain of antibodies results in products being heterogeneous. In order to avoid said product heterogeneity, the respective codon of the C-terminal lysine of the heavy antibody chain is deleted using recombinant DNA technology. Said optimized antibodies result in an increased product titer than in the wild type. In addition, said optimized antibodies are advantageous in the purification process as a result of an improved elution behavior due to the reduced charge heterogeneity.
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2.发明授权
公开(公告)号：EP2032230B1
公开(公告)日：2014-04-02
申请号：EP07765375.6
申请日：2007-06-12
CPC分类号： G01N30/28 , B01D15/08 , B01D15/203 , G01N30/466 , G01N30/8658 , G01N2030/009
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3.发明公开
公开(公告)号：EP2032230A1
公开(公告)日：2009-03-11
申请号：EP07765375.6
申请日：2007-06-12
CPC分类号： G01N30/28 , B01D15/08 , B01D15/203 , G01N30/466 , G01N30/8658 , G01N2030/009
摘要： The invention relates to a process for finding suitable parameters for the chromatographic purification of biomolecules. The process consists of equilibration, loading, washing and elution steps, wherein this sequence of steps is carried out in the partial batch process. The parameters determined in small, preferably a plurality, of parallel batches give information on the chromatography conditions under which a given biomolecule may be optimally purified by column chromatography, if appropriate even on a relatively large scale.
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