- 专利标题: METHOD FOR AMPLIFICATION OF NUCLEOTIDE SEQUENCE
- 专利标题(中): 核苷酸放大
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申请号: EP07767254.1申请日: 2007-06-20
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公开(公告)号: EP2050819B1公开(公告)日: 2012-03-28
- 发明人: NAKASHIMA, Katsunori , OHISO, Isao
- 申请人: Nishikawa Rubber Co., Ltd.
- 申请人地址: 2-8, Misasamachi 2-chome, Nishi-ku Hiroshima-shi, Hiroshima 733-8510 JP
- 专利权人: Nishikawa Rubber Co., Ltd.
- 当前专利权人: Nishikawa Rubber Co., Ltd.
- 当前专利权人地址: 2-8, Misasamachi 2-chome, Nishi-ku Hiroshima-shi, Hiroshima 733-8510 JP
- 代理机构: Jappy, John William Graham
- 优先权: JP2006203414 20060726; JP2007136392 20070523
- 国际公布: WO2008013010 20080131
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12Q1/68
摘要:
Disclosed are: a method for the selective amplification of a target nucleic acid, which is useful as a method for the synthesis of a nucleic acid; and a method for the detection of a nucleic acid by using the method. Specifically, disclosed are: a nucleotide sequence amplification method (i.e., an EVA (endonuclease V-dependent amplification) method) for selectively amplifying a target nucleic acid in a sample by using an oligonucleotide primer having a nucleotide base capable of being recognized by endonuclease V, endonuclease V and a DNA polymerase having a chain-substitution activity; and a method for the detection of a nucleic acid by using the method.
公开/授权文献
- EP2050819A1 METHOD FOR AMPLIFICATION OF NUCLEOTIDE SEQUENCE 公开/授权日:2009-04-22
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