发明公开
EP2087131A1 METHOD FOR MEASURING THE CONCENTRATION OF TRANSIENT PROTEOLYTIC ACTIVITY IN COMPOSITE BIOLOGICAL MEDIA CONTAINING CELLS
有权
法测量瞬态蛋白水解活性的浓度，细胞一种含有复合生物MEDIA
- 专利标题： METHOD FOR MEASURING THE CONCENTRATION OF TRANSIENT PROTEOLYTIC ACTIVITY IN COMPOSITE BIOLOGICAL MEDIA CONTAINING CELLS
- 专利标题（中）： 法测量瞬态蛋白水解活性的浓度，细胞一种含有复合生物MEDIA
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申请号： EP07819552.6申请日： 2007-11-02
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公开(公告)号： EP2087131A1公开(公告)日： 2009-08-12
- 发明人: GIESEN, Peter L., A. , TAPPENDEN, Kerry , JONES, Wynne
- 申请人： Thrombinoscope B.V.
- 申请人地址： Oude Maasstraat 47 6229 BC Maastricht NL
- 专利权人： Thrombinoscope B.V.
- 当前专利权人： Thrombinoscope B.V.
- 当前专利权人地址： Oude Maasstraat 47 6229 BC Maastricht NL
- 代理机构： Huygens, Arthur Victor
- 优先权： EP06022813 20061102
- 国际公布： WO2008052795 20080508
- 主分类号： C12Q1/37
- IPC分类号： C12Q1/37 ; C12Q1/56
摘要：
A method is provided for determining in real time the course of thrombin activity in a sample of clotting blood or plasma as it appears in and disappears from the sample which comprises adding a signal substrate to said sample, said signal substrate causing a detectable signal related to the amount of conversion product formed upon reaction by the generated proteolytic activity, monitoring the signal development in time in said sample to provide a curve, and mixing said sample frequently so that clot formation occurs in a dense manner such that the majority of the sample remains fluid and that cell precipitation is inhibited, wherein said monitoring and mixing steps are repeated and performed in an alternate way.
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