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1.发明公开METHOD FOR MEASURING THE CONCENTRATION OF TRANSIENT PROTEOLYTIC ACTIVITY IN COMPOSITE BIOLOGICAL MEDIA CONTAINING CELLS 有权法测量瞬态蛋白水解活性的浓度，细胞一种含有复合生物MEDIA
公开(公告)号：EP2087131A1
公开(公告)日：2009-08-12
申请号：EP07819552.6
申请日：2007-11-02
申请人： Thrombinoscope B.V.
摘要： A method is provided for determining in real time the course of thrombin activity in a sample of clotting blood or plasma as it appears in and disappears from the sample which comprises adding a signal substrate to said sample, said signal substrate causing a detectable signal related to the amount of conversion product formed upon reaction by the generated proteolytic activity, monitoring the signal development in time in said sample to provide a curve, and mixing said sample frequently so that clot formation occurs in a dense manner such that the majority of the sample remains fluid and that cell precipitation is inhibited, wherein said monitoring and mixing steps are repeated and performed in an alternate way.
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2.发明授权METHOD FOR MEASURING THE CONCENTRATION OF TRANSIENT PROTEOLYTIC ACTIVITY IN COMPOSITE BIOLOGICAL MEDIA CONTAINING CELLS 有权法测量瞬态蛋白水解活性的浓度，细胞一种含有复合生物MEDIA
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