The present invention relates to a method for detecting analytes of biological samples, said method comprising the following method steps of: a) providing a reversible binding partner 1 which is immobilized on a solid phase and to which an analyte binder is reversibly bound via a reversible binding partner 2 which is bound to the analyte binder, wherein the analyte binder is immobilized by binding the reversible binding partners 1 and 2, b) adding the biological sample and binding the analyte to the reversibly immobilized analyte binder in the event that the biological sample contains the analyte, c) separating the biological sample, d) adding a stripping buffer which dissolves the bond between the reversible binding partners 1 and 2, wherein the bond between the analyte and the analyte binder optionally remains, and e) detecting the analyte in the stripping buffer in the event that the biological sample contains the analyte or determining the absence of the analyte in the event that the biological sample does not contain the analyte.