发明授权
- 专利标题: Capsular polysaccharide solubilisation and combination vaccines
- 专利标题(中): 荚膜多糖增溶和联合疫苗
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申请号: EP09075005.0申请日: 2002-06-20
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公开(公告)号: EP2184071B1公开(公告)日: 2016-08-31
- 发明人: Costantino, Paolo
- 申请人: GlaxoSmithKline Biologicals SA
- 申请人地址: Rue de l'Institut 89 1330 Rixensart BE
- 专利权人: GlaxoSmithKline Biologicals SA
- 当前专利权人: GlaxoSmithKline Biologicals SA
- 当前专利权人地址: Rue de l'Institut 89 1330 Rixensart BE
- 代理机构: Evans, Stephen John Eves
- 优先权: GB0115176 20010620
- 主分类号: A61K39/095
- IPC分类号: A61K39/095 ; A61K39/385 ; A61P31/04 ; C12P19/04
摘要:
Precipitated bacterial capsular polysaccharides can be efficiently re-solubilised using alcohols as solvents. The invention provides a process for purifying a bacterial capsular polysaccharide, comprising the steps of (a) precipitation of said polysaccharide, followed by (b) solubilisation of the precipitated polysaccharide using ethanol. CTAB can be used for step (a). The material obtained, preferably following hydrolysis and sizing, can be conjugated to a carrier protein and formulated as a vaccine. Also, in vaccines comprising saccharides from both serogroups A and C, the invention provides that the ratio (w/w) of MenA saccharide : MenC saccharide is >1.
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