The invention provides a set of nucleotides against HIV infection and their application for treatment and prevention of AIDS. The sequences of the nucleotides were shown in the sequences table. The invention was superior to the similar technology as following: High conserved RNA sequences were obtained by compare all the published HIV genome sequences. Double strand RNA sequences derived from the sequences can effectively inhibit the expression of target HIV gene. HIV gene expression can also be inhibited by double strand hairpin-like RNA transcribed in the cells harboring recombinant plasmid which contain the DNA sequences encoding the RNA sequences. Further, HIV gene expression can be knocked down by in cell transcribed double strand RNA corresponding to the RNA sequences encoded by recombinant adenovirus associated virus.