发明公开
EP2279241A4 DEVELOPMENT OF STRAINS OF THE THERMOTOLERANT YEAST HANSENULA POLYMORPHA CAPABLE OF ALCOHOLIC FERMENTATION OF STARCH AND XYLAN BY EXPRESSION OF STARCH AND XYLAN DEGRADING ENZYMES
审中-公开
耐热的酵母汉逊酵母的为力量的酒精发酵和木聚糖串BY表达的淀粉酶XYLANABBAUENDEN发展
- 专利标题: DEVELOPMENT OF STRAINS OF THE THERMOTOLERANT YEAST HANSENULA POLYMORPHA CAPABLE OF ALCOHOLIC FERMENTATION OF STARCH AND XYLAN BY EXPRESSION OF STARCH AND XYLAN DEGRADING ENZYMES
- 专利标题(中): 耐热的酵母汉逊酵母的为力量的酒精发酵和木聚糖串BY表达的淀粉酶XYLANABBAUENDEN发展
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申请号: EP09743560申请日: 2009-05-06
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公开(公告)号: EP2279241A4公开(公告)日: 2012-05-30
- 发明人: ABBAS CHARLES , SIBIRNY ANDRIY , VORONOVSKY ANDRIY Y
- 申请人: ARCHER DANIELS MIDLAND CO
- 专利权人: ARCHER DANIELS MIDLAND CO
- 当前专利权人: ARCHER DANIELS MIDLAND CO
- 优先权: US5068508 2008-05-06
- 主分类号: C12N1/19
- IPC分类号: C12N1/19 ; C12N15/56 ; C12P7/06 ; C12R1/01
摘要:
Genes SWA2 and GAM1 from the yeast, Schwanniomyces occidentalis, encoding α-amylase and glucoamylase, respectively, were cloned and expressed in H. polymorpha. The expression was achieved by integration of the SWA2 and GAM1 genes into the chromosome of H. polymorpha under operably linked to a strong constitutive promoter of the H. polymorpha-glyceraldehyde-3-phosphate dehydrogenase gene (HpGAP. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus Trichoderma resee, and a xlnD gene coding for &bgr;-xylosidase was obtained from the fungus Aspergillus niger. Co-expression of these genes was also achieved by integration into the H. polymorpha chromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in a H. polymorpha strain that overexpresses a pyruvate decarboxylase gene further improved ethanol production.
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