公开(公告)号：EP4311555A1

公开(公告)日：2024-01-31

申请号：EP22775729.1

申请日：2022-03-23

申请人： JCR Pharmaceuticals Co., Ltd.

发明人： YASUKAWA, Hidehito ,  MURASE, Hiroaki ,  YAMAGUCHI, Yuka ,  OKABE, Shinji

IPC分类号： A61K38/43 ,  A61K47/34 ,  A61J1/05 ,  A61K47/65 ,  A61K9/08 ,  A61K47/68 ,  A61K9/19 ,  A61P3/00 ,  A61K38/47 ,  A61P43/00 ,  A61K39/395 ,  C07K16/28 ,  A61K45/00 ,  C07K16/46 ,  A61K47/02 ,  C12N9/24 ,  A61K47/10 ,  C12N15/13 ,  A61K47/12 ,  C12N15/56 ,  A61K47/18

摘要： Disclosed is a stable aqueous pharmaceutical composition or lyophilized pharmaceutical composition comprising a protein as the active ingredient. The pharmaceutical composition comprises a protein having physiological activity and two different nonionic surfactants, including polysorbate 80 and polyoxyethylene(160) polyoxypropylene(30) glycol as the nonionic surfactants, for example, and as optional components, sodium chloride as a neutral salt, sucrose as a disaccharide and citrate buffer as a buffering agent.

公开(公告)号：EP4299756A1

公开(公告)日：2024-01-03

申请号：EP22767112.0

申请日：2022-03-08

申请人： JCR Pharmaceuticals Co., Ltd.

发明人： KAKIMOTO, Shinji ,  FUKUI, Tsuyoshi ,  HATANO, Yukichi ,  KOTANI, Ayaka ,  MIURA, Takuya ,  ISHIGURO, Toshifumi

IPC分类号： C12P21/02 ,  C07K1/16 ,  C07K16/28 ,  C07K19/00 ,  C12N9/24 ,  C12N15/13 ,  C12N15/56 ,  C12N15/62

摘要： Disclosed is a method for producing a fusion protein in which an antibody and a lysosomal enzyme are fused. The method is a method for producing a fusion protein in which an antibody and a human lysosomal enzyme are fused, the method including: (a) culturing mammalian cells producing the fusion protein in a serum-free medium to secrete the fusion protein in a culture medium; (b) collecting a culture supernatant by removing the mammalian cells from the culture medium; and (c) purifying the fusion protein from the culture supernatant by using a column chromatography using a material to which a substance having affinity for the antibody is bound as a solid phase, an anion exchange column chromatography, a cation exchange column chromatography, and a size exclusion column chromatography.

公开(公告)号：EP4209506A1

公开(公告)日：2023-07-12

申请号：EP21864357.5

申请日：2021-09-01

申请人： Daiichi Sankyo Company, Limited

发明人： NISHIZAWA Hanako ,  ONO Yasunori ,  IWAMOTO Mitsuhiro

IPC分类号： C07K16/00 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/54 ,  C12N15/56 ,  C12N15/63 ,  C12N9/10 ,  C12N9/24 ,  C12P21/02 ,  C12P21/08

摘要： The present invention provides endo-β-N-acetylglucosaminidase (Endo-Si) cloning from a strain belonging to Streptococcus iniae and a mutant enzyme thereof, a gene encoding the enzyme, a recombinant plasmid, a transformant obtained by transformation of a cell by the plasmid and use thereof, and a method for producing e.g., a sugar chain remodeled antibody using the enzyme. A polypeptide having an amino acid sequence at amino acid positions 34 to 928 in SEQ ID NO: 2 or an amino acid sequence having the same amino acid sequence except containing one or mutations at more amino acid positions selected from the group consisting of amino acids at position 241 (D241), 190 (T190), 311 (Q311) and 360 (E360), said polypeptide exhibiting a sugar chain hydrolysis activity and/or transglycosylation activity.

公开(公告)号：EP3234143B1

公开(公告)日：2023-06-28

申请号：EP15869363.0

申请日：2015-12-18

发明人： PENG, Wei ,  PEDERSEN, Ninfa Rangel ,  PETTERSSON, Dan ,  EKLOEF, Jens Magnus ,  NYMAND-GRARUP, Soren ,  PALMEN, Lorena G. ,  MONRAD, Rune Nygaard ,  SPODSBERG, Nikolaj ,  STRINGER, Mary Ann ,  BLOM, Charlotte ,  KIEMER, Lars ,  KROGH, Kristian Bertel Romer M. ,  SALOMON, Jesper

IPC分类号： C12N15/56 ,  C12N9/24

公开(公告)号：EP3152303B1

公开(公告)日：2021-11-24

申请号：EP15803007.2

申请日：2015-06-05

发明人： HOWELL, Lynne ,  BAKER, Perrin ,  ALNABELSEYA, Noor ,  BAMFORD, Natalie ,  LITTLE, Dustin ,  SHEPPARD, Donald ,  SNARR, Brendan ,  LEE, Mark Jae

IPC分类号： C12N9/24 ,  A01N63/00 ,  A01P1/00 ,  A01P3/00 ,  A61K38/16 ,  A61L2/16 ,  A61L27/22 ,  A61L27/54 ,  A61L31/10 ,  A61L31/16 ,  A61L31/00 ,  C12N15/56 ,  C12N15/63 ,  C12N15/80 ,  C12N7/01

公开(公告)号：EP3475419B1

公开(公告)日：2021-09-01

申请号：EP17814823.5

申请日：2017-06-22

发明人： LOPONEN, Jussi ,  MIKOLA, Markku ,  SIBAKOV, Juhani

IPC分类号： C12N9/24 ,  C12N15/56 ,  C12N15/63 ,  A23L5/20 ,  A21D8/04 ,  A23L7/104

公开(公告)号：EP3000887B1

公开(公告)日：2019-10-02

申请号：EP15189565.3

申请日：2005-07-05

申请人： Novozymes A/S

发明人： ANDERSEN, Carsten ,  OESTDAL, Henrik ,  SKAGERLIND, Peter

IPC分类号： C12N15/56 ,  C12N9/28 ,  C12N15/63 ,  C12N1/15 ,  C12N1/21 ,  C11D3/386 ,  D06M16/00

公开(公告)号：EP2773656B1

公开(公告)日：2019-06-19

申请号：EP12846110.0

申请日：2012-10-31

申请人： Novozymes, Inc.

发明人： ZHANG, Yu ,  DUAN, Junxin ,  LUI, Ye ,  TANG, Lan ,  SHAGHASI, Tarana

IPC分类号： C07K14/37 ,  C12N9/42 ,  C12N15/56 ,  C12N15/63 ,  C12N5/10 ,  C12P19/14 ,  A01H5/00

公开(公告)号：EP3421596A1

公开(公告)日：2019-01-02

申请号：EP17755837.6

申请日：2017-02-24

申请人： Nanjing Bestzyme Bio-engineering Co. Ltd.

发明人： FAN, Yan ,  DU, Xiuzhen ,  HAO, Minghui ,  SUN, Yan ,  HUANG, Ke ,  LI, Feng

IPC分类号： C12N9/28 ,  C12N15/56 ,  C12N15/75 ,  C12N1/21 ,  C12P19/14 ,  C12P19/04 ,  C12R1/10

摘要： Provided is an α amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent α amylase, and at the same time, same is an α amylase maintaining the capability of the parent to hydrolyse α-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of α amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100°C..

公开(公告)号：EP3402872A1

公开(公告)日：2018-11-21

申请号：EP17738281.9

申请日：2017-01-11

申请人： 3PLW Ltd.

发明人： SHAPIRA, Tal ,  ORANIM, Amir

IPC分类号： C12N1/21 ,  C12N1/22 ,  C12N9/28 ,  C12N9/42 ,  C12N15/31 ,  C12N15/56 ,  C12N15/74

CPC分类号： C12P7/56 ,  C12N1/38 ,  C12N9/2411 ,  C12N9/2437 ,  C12N9/2477

摘要： Lactic acid (LA)-utilizing bacteria are provided, genetically modified to express and optionally secrete polysaccharide-degrading enzymes, such as cellulases, hemicellulases and amylases, and uses thereof. The polysaccharide-degrading enzymes are advantageous for processing organic waste so that the organic waste can be used as a substrate in industrial fermentation processes, particularly industrial production of discrete lactic acid enantiomer(s). Vectors and constructs useful for genetically modifying the LA- utilizing bacteria are also provided.