发明公开
- 专利标题： Fusion proteins useful for detecting analytes
- 专利标题（中）： 融合蛋白用于检测分析物
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申请号： EP10182215.3申请日： 2002-09-13
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公开(公告)号： EP2284195A9公开(公告)日： 2013-03-27
- 发明人: Fehr, Marcus , Lalonde, Sylvie , Frommer, Wolf-Bernd
- 申请人： Carnegie Institution Of Washington
- 申请人地址： 1530 P Street NW Washington DC 20005 US
- 专利权人： Carnegie Institution Of Washington
- 当前专利权人： Carnegie Institution Of Washington
- 当前专利权人地址： 1530 P Street NW Washington DC 20005 US
- 代理机构： Lipscombe, Martin John
- 优先权： EP01122301 20010918; EP02007373 20020408
- 主分类号： C07K19/00
- IPC分类号： C07K19/00 ; C07K14/195 ; C07K14/435 ; C12N15/62 ; C12N15/63 ; G01N33/542
摘要：
Disclosed is a fusion protein comprising:
(a) two detection portions, wherein
(i) the first detection portion is an energy-emitting protein portion and the second detection portion is a fluorescent protein portion;
or
(ii) the two detection portions are portions of a split fluorescent protein; and
(b) a periplasmic binding protein (PBP) portion between said two detection portions, which undergoes a conformational change upon binding of a compound;
wherein said conformational change results in a change of the energy emitted by said two detection portions.
(a) two detection portions, wherein
(i) the first detection portion is an energy-emitting protein portion and the second detection portion is a fluorescent protein portion;
or
(ii) the two detection portions are portions of a split fluorescent protein; and
(b) a periplasmic binding protein (PBP) portion between said two detection portions, which undergoes a conformational change upon binding of a compound;
wherein said conformational change results in a change of the energy emitted by said two detection portions.
公开/授权文献
- EP2284195A1 Fusion proteins useful for detecting analytes 公开/授权日：2011-02-16
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