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公开(公告)号：EP2427471A2
公开(公告)日：2012-03-14
申请号：EP10772690.3
申请日：2010-05-04
发明人： FROMMER, Wolf, B. , LALONDE, Sylvie
CPC分类号： C12N15/8279 , C07K14/415 , C07K14/43545 , C07K14/47 , C12N15/8218 , C12N15/8245 , C12N15/8246
摘要： A novel class of transporter protein, referred to as SWEET, GLUE or Glü, is disclosed. These transporters provide a novel system for the transportation of sugars across membranes within a cell and between the inside and outside of a cell. Such transporters are useful for understanding and altering the sugar concentration within certain organs of an organism, and within certain organelles within the cell. These transporters are also useful in protecting plants from a pathogen attack.
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公开(公告)号：EP1805200B1
公开(公告)日：2012-01-04
申请号：EP05812420.7
申请日：2005-10-14
CPC分类号： G01N33/542 , G01N33/9406 , G01N2500/00
摘要： Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotranmitter concentrations in vivo and in culture.
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公开(公告)号：EP2267131A1
公开(公告)日：2010-12-29
申请号：EP10177654.0
申请日：1998-12-21
发明人： Fire, Andrew , Xu, Siqun , Montgomery, Mary K. , Kostas, Stephen A. , Timmons, Lisa , Tabara, Hiroaki , Driver, Samuel E. , Mello, Crag C.
CPC分类号： C12N15/8285 , A01K2217/05 , A01K2227/703 , A61K31/7105 , A61K31/713 , C12N15/113 , C12N15/8218 , C12N15/85 , C12N2310/11 , C12N2310/14 , C12N2310/321 , C12N2310/50 , C12N2310/53
摘要： A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo . The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.
摘要翻译： 提供了将RNA引入活细胞以抑制该细胞中靶基因的基因表达的方法。 该方法可以离体或体内实施。 RNA具有双链结构的区域。 抑制是序列特异性的，因为RNA的双链体区域和靶基因的一部分的核苷酸序列是相同的。 本发明不同于现有技术通过反义或三链方法的基因表达干扰。
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公开(公告)号：EP1664373A2
公开(公告)日：2006-06-07
申请号：EP04786066.3
申请日：2004-07-14
IPC分类号： C23C16/26
CPC分类号： C30B33/02 , C01B32/25 , C23C16/274 , C23C16/277 , C23C16/279 , C30B25/105 , C30B29/04 , C30B33/00 , G11B2220/20 , G11B2220/65 , H04N5/76 , H04N5/765 , H04N5/775 , H04N5/781 , H04N9/8042 , H04N9/8205
摘要： A single crystal diamond grown by microwave plasma chemical vapor deposition annealed at pressures in excess of 4.0 GPa and heated to temperature in excess of 1500 degrees C. that has a hardness of greater than 120 GPa. A method for manufacture a hard single crystal diamond includes growing a single crystal diamond and annealing the single crystal diamond at pressures in excess of 4.0 GPa and a temperature in excess of 1500 degrees C. to have a hardness in excess of 120 GPa.
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公开(公告)号：EP1444390A2
公开(公告)日：2004-08-11
申请号：EP02797071.4
申请日：2002-11-07
CPC分类号： C30B25/105 , C30B25/12 , C30B29/04 , Y10T117/1004 , Y10T117/1008 , Y10T117/1016 , Y10T117/108
摘要： An apparatus for producing diamond in a deposition chamber including a heat-sinking holder for holding a diamond and for making thermal contact with a side surface of the diamond adjacent to an edge of a growth surface of the diamond, a noncontact temperature measurement device positioned to measure temperature of the diamond across the growth surface of the diamond and a main process controller for receiving a temperature measurement from the noncontact temperature measurement device and controlling temperature of the growth surface such that all temperature gradients across the growth surface are less than 20° C. The method for producing diamond includes positioning diamond in a holder such that a thermal contact is made with a side surface of the diamond adjacent to an edge of a growth surface of the diamond, measuring temperature of the growth surface of the diamond to generate temperature measurements, controlling temperature of the growth surface based upon the temperature measurements, and growing single-crystal diamond by microwave plasma chemical vapor deposition on the growth surface, wherein a growth rate of the diamond is greater than 1 micrometer per hour.
摘要翻译： 一种用于在沉积室中制造金刚石的设备，所述设备包括用于保持金刚石并且用于与金刚石的与金刚石的生长表面的边缘相邻的金刚石的侧表面进行热接触的加热/下沉保持器，非接触式温度测量装置， 测量穿过金刚石生长表面的金刚石的温度，以及主处理控制器，用于接收来自非接触式温度测量装置的温度测量结果并控制生长表面的温度，使得生长表面上的所有温度梯度小于20℃ 。制造钻石的方法包括将金刚石定位在保持器中，使得与金刚石的与金刚石的生长表面的边缘相邻的侧表面形成热接触，测量金刚石的生长表面的温度以产生温度 测量，基于温度测量来控制生长表面的温度 在生长表面上通过微波等离子体化学气相沉积生长单晶金刚石和生长单晶金刚石，其中金刚石的生长速率大于1微米每小时。
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公开(公告)号：EP1009220A1
公开(公告)日：2000-06-21
申请号：EP97908648.5
申请日：1997-02-06
CPC分类号： C12N9/0071 , C12N9/0073 , C12N9/0083 , C12N15/8222 , C12N15/8247
摘要： This invention relates to plant fatty acid hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acid hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.
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公开(公告)号：EP0359589A2
公开(公告)日：1990-03-21
申请号：EP89309423.5
申请日：1989-09-15
发明人： Schwartz, David C.
IPC分类号： G01N27/26
CPC分类号： G01N27/44773 , G01N27/44704 , G01N27/44721 , G01N2015/0288 , G01N2015/105 , G01N2015/1062 , G01N2015/1081 , G01N2015/1486 , G01N2015/149
摘要： Disclosed herein is a method of electrophoretically separating particles by the application to pulsed field electrophoresis of a pulsing routine in which the pulses are modulated in such a way as to define the effective field angle of the particles undergoing separation as determined by the vector sum of the pulses. In one embodiment of the invention, pulse times are modulated to define the orientation of the particles undergoing separation. In a second embodiment, the intensities of the pulses are modulated to define the orientation of the particles. The method of the invention allows the simple and dynamic determination of effective field angle by pulsing routine rather than by placement of an electrode array. In addition, the invention makes it possible for the first time to translate particles undergoing separation incrementally through a gel matrix.
摘要翻译： 本文公开了一种通过应用于脉冲程序的脉冲场电泳的电泳分离颗粒的方法，其中脉冲被调制以限定经历分离的颗粒的有效视场角，如由 脉冲。 在本发明的一个实施例中，调制脉冲时间以限定经历分离的颗粒的取向。 在第二实施例中，调制脉冲的强度以限定颗粒的取向。 本发明的方法允许通过脉冲程序而不是通过放置电极阵列简单且动态地确定有效视场角。 此外，本发明使得可以首次平移通过凝胶基质逐渐分离的颗粒。
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8.发明授权METHOD TO MEASURE THE METABOLIC RATE OR RATE OF GLUCOSE CONSUMPTION OF CELLS OR TISSUES WITH HIGH SPATIOTEMPORAL RESOLUTION USING A GLUCOSE NANOSENSOR 有权使用葡萄糖纳米传感器测量具有高时间分辨率的细胞或组织的葡萄糖消耗的代谢率或速率的方法
公开(公告)号：EP2588854B1
公开(公告)日：2017-05-31
申请号：EP10854225.9
申请日：2010-06-30
IPC分类号： G01N33/574 , G01N33/00 , C12Q1/02 , C12Q1/54 , G01N33/66
CPC分类号： C12Q1/54 , C12Q1/025 , G01N33/574 , G01N33/66 , G01N2500/10
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9.发明公开METHOD TO MEASURE THE METABOLIC RATE OR RATE OF GLUCOSE CONSUMPTION OF CELLS OR TISSUES WITH HIGH SPATIOTEMPORAL RESOLUTION USING A GLUCOSE NANOSENSOR 有权法测量细胞的代谢或葡萄糖消耗速度或组织与高时间分辨率上国土葡萄糖传感器NANO的基础上的
公开(公告)号：EP2588854A1
公开(公告)日：2013-05-08
申请号：EP10854225.9
申请日：2010-06-30
IPC分类号： G01N33/00
CPC分类号： C12Q1/54 , C12Q1/025 , G01N33/574 , G01N33/66 , G01N2500/10
摘要： A method to measure the metabolic rate or rate of glucose consumption of cells or tissues with high spatiotemporal resolution using a glucose nanosensor is disclosed. The method generally includes providing single cells or cell populations, cells in suspension or adherent, in a cell culture, a tissue culture, a mixed cell culture, a tissue explant, or in animal tissues in vivo for the measurement of glucose metabolic rate; expressing a glucose sensor in individual cells; calibrating the sensor in controlled conditions; disrupting the steady-state of glucose entering the cell; recording the output from the sensor and calculating the corresponding glucose concentration at different times; and determining the glucose metabolic rate. The method can be applied for the screening of molecules with pharmacological potential, determination of glucose rate of cancerous cells, tissue physiology and biochemistry research.
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10.发明公开BRASSINOSTEROID REGULATED KINASES (BRKS) THAT MEDIATE BRASSINOSTEROID SIGNAL TRANSDUCTION AND USES THEREOF 审中-公开油菜素激酶（BRKS）FOR BRASSINOSTEROIDSIGNALTRANSDUKTION和使用的放置
公开(公告)号：EP2315770A2
公开(公告)日：2011-05-04
申请号：EP09800658.8
申请日：2009-07-21
发明人： WANG, Zhi-Yong , TANG, Wenqiang
IPC分类号： C07H21/04
CPC分类号： C12N15/8261 , C12N9/1205 , Y02A40/146
摘要： The present invention identifies a novel family of kinases regulated by brassinosteroids, referred to as BRKs (brassinosteroid regulated kinases) or BSKs (brassinosteroid signaling kinases). The present invention provides methods for modulating the response of a plant cell to a brassinosteroid using BRKs.
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