发明公开
- 专利标题： Method for the amplificiation of HLA class I alleles
- 专利标题（中）： 用于Ⅰ类HLA等位基因的扩增方法
-
申请号： EP10182224.5申请日： 2000-04-05
-
公开(公告)号： EP2319942A2公开(公告)日： 2011-05-11
- 发明人: De Canck, Ilse , Rombout, Annelies , Rossau, Rudi
- 申请人： Innogenetics N.V.
- 申请人地址： Industriepark Zwijnaarde 7, Box 4 9052 Ghent BE
- 专利权人： Innogenetics N.V.
- 当前专利权人： Innogenetics N.V.
- 当前专利权人地址： Industriepark Zwijnaarde 7, Box 4 9052 Ghent BE
- 优先权： US138614P 19990611; EP99870068 19990409
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention relates to a method and to specific primers for the locus-specific, separate amplification of exon 2, exon 3 and/or exon 4 of HLA-A, HLA-B or HLA-C alleles, making use of at least one primer set wherein:
- for the amplification of exon 2, the reverse primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 3, the forward primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C and/or the reverse primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 4, the forward primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C.
In accordance, the present invention provides an improved method for the typing or subtyping of HLA Class I alleles making use of the amplification method of the invention.
- for the amplification of exon 2, the reverse primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 3, the forward primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C and/or the reverse primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 4, the forward primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C.
In accordance, the present invention provides an improved method for the typing or subtyping of HLA Class I alleles making use of the amplification method of the invention.
公开/授权文献
- EP2319942B1 Method for the amplificiation of HLA class I alleles 公开/授权日：2013-08-14
信息查询
