公开(公告)号：EP2314715B1

公开(公告)日：2013-08-14

申请号：EP10008785.7

申请日：2000-04-05

申请人： Innogenetics N.V.

发明人： De Canck, Ilse ,  Rombout, Annelies ,  Rossau, Rudi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/172

公开(公告)号：EP2314715A3

公开(公告)日：2012-04-18

申请号：EP10008785.7

申请日：2000-04-05

申请人： Innogenetics N.V.

发明人： De Canck, Ilse ,  Rombout, Annelies ,  Rossau, Rudi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/172

摘要： The present invention relates to a method and to specific primers for the locus-specific, separate amplification of exon 2, exon 3 and/or exon 4 of HLA-A, HLA-B or HLA-C alleles, making use of at least one primer set wherein:
- for the amplification of exon 2, the reverse primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 3, the forward primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C and/or the reverse primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 4, the forward primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C.
In accordance, the present invention provides an improved method for the typing or subtyping of HLA Class I alleles making use of the amplification method of the invention.

公开(公告)号：EP2319942B1

公开(公告)日：2013-08-14

申请号：EP10182224.5

申请日：2000-04-05

申请人： Innogenetics N.V.

发明人： De Canck, Ilse ,  Rombout, Annelies ,  Rossau, Rudi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/172

公开(公告)号：EP2319942A3

公开(公告)日：2012-04-18

申请号：EP10182224.5

申请日：2000-04-05

申请人： Innogenetics N.V.

发明人： De Canck, Ilse ,  Rombout, Annelies ,  Rossau, Rudi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/172

摘要： The present invention relates to a method and to specific primers for the locus-specific, separate amplification of exon 2, exon 3 and/or exon 4 of HLA-A, HLA-B or HLA-C alleles, making use of at least one primer set wherein:
- for the amplification of exon 2, the reverse primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 3, the forward primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C and/or the reverse primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 4, the forward primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C.
In accordance, the present invention provides an improved method for the typing or subtyping of HLA Class I alleles making use of the amplification method of the invention.

摘要翻译： 本发明涉及用于HLA-A，HLA-B或HLA-C等位基因的外显子2，外显子3和/或外显子4的基因座特异性，单独扩增的方法和特异性引物，利用至少一个 其中： - 为了扩增外显子2，反向引物与HLA-A，HLA-B或HLA-C各自的内含子2中的基因座特异性靶序列特异性杂交; - 为了扩增外显子3，正向引物与HLA-A，HLA-B或HLA-C各自的内含子2中的基因座特异性靶序列特异性杂交和/或反向引物与基因座特异性靶特异性杂交 序列分别为HLA-A，HLA-B或HLA-C的内含子3; - 为了扩增外显子4，正向引物与HLA-A，HLA-B或HLA-C各自的内含子3中的基因座特异性靶序列特异性杂交。 因此，本发明提供了利用本发明的扩增方法对HLA I类等位基因进行分型或亚型分型的改进方法。

公开(公告)号：EP2319942A2

公开(公告)日：2011-05-11

申请号：EP10182224.5

申请日：2000-04-05

申请人： Innogenetics N.V.

发明人： De Canck, Ilse ,  Rombout, Annelies ,  Rossau, Rudi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/172

摘要： The present invention relates to a method and to specific primers for the locus-specific, separate amplification of exon 2, exon 3 and/or exon 4 of HLA-A, HLA-B or HLA-C alleles, making use of at least one primer set wherein:
- for the amplification of exon 2, the reverse primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 3, the forward primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C and/or the reverse primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 4, the forward primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C.
In accordance, the present invention provides an improved method for the typing or subtyping of HLA Class I alleles making use of the amplification method of the invention.

公开(公告)号：EP2314715A2

公开(公告)日：2011-04-27

申请号：EP10008785.7

申请日：2000-04-05

申请人： Innogenetics N.V.

发明人： De Canck, Ilse ,  Rombout, Annelies ,  Rossau, Rudi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q2600/172

摘要： The present invention relates to a method and to specific primers for the locus-specific, separate amplification of exon 2, exon 3 and/or exon 4 of HLA-A, HLA-B or HLA-C alleles, making use of at least one primer set wherein:
- for the amplification of exon 2, the reverse primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 3, the forward primer specifically hybridizes to a locus-specific target sequence in intron 2 of respectively HLA-A, HLA-B or HLA-C and/or the reverse primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C;
- for the amplification of exon 4, the forward primer specifically hybridizes to a locus-specific target sequence in intron 3 of respectively HLA-A, HLA-B or HLA-C.
In accordance, the present invention provides an improved method for the typing or subtyping of HLA Class I alleles making use of the amplification method of the invention.

摘要翻译： 本发明涉及用于HLA-A，HLA-B或HLA-C等位基因的外显子2，外显子3和/或外显子4的基因座特异性，单独扩增的方法和特异性引物，利用至少一个 其中： - 为了扩增外显子2，反向引物与HLA-A，HLA-B或HLA-C各自的内含子2中的基因座特异性靶序列特异性杂交; - 为了扩增外显子3，正向引物与HLA-A，HLA-B或HLA-C各自的内含子2中的基因座特异性靶序列特异性杂交和/或反向引物与基因座特异性靶特异性杂交 序列分别为HLA-A，HLA-B或HLA-C的内含子3; - 为了扩增外显子4，正向引物与HLA-A，HLA-B或HLA-C各自的内含子3中的基因座特异性靶序列特异性杂交。 因此，本发明提供了利用本发明的扩增方法对HLA I类等位基因进行分型或亚型分型的改进方法。