An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. The present invention provides a method of forming a two-dimensional distribution image of a target constituent based on information on the mass of constituents of the tissue section wherein, as the internal standard material, any one of actin, tubulin and GAPDH is used in the intracellular region, one of histone and nucleic acid is used in the nuclear region, and one of albumin and cytokine is used in the extracellular region.