公开(公告)号：EP2635597A1

公开(公告)日：2013-09-11

申请号：EP11804829.7

申请日：2011-11-28

申请人： Canon Kabushiki Kaisha

发明人： MURAYAMA, Yohei ,  HASHIMOTO, Hiroyuki ,  KOMATSU, Manabu

IPC分类号： C07K14/475 ,  G01N33/574 ,  G01N33/68

CPC分类号： G01N33/57415 ,  C07K14/4756 ,  G01N33/6848 ,  G01N2333/4756

摘要： The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500.

公开(公告)号：EP1518119A1

公开(公告)日：2005-03-30

申请号：EP03738557.2

申请日：2003-06-27

申请人： Canon Kabushiki Kaisha

发明人： HASHIMOTO, Hiroyuki ,  OKAMOTO, Tadashi ,  ISHII, Mie

IPC分类号： G01N33/53 ,  G01N37/00

CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00693 ,  B01J2219/00707 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： ABSTRACT To provide a technology for obtaining a probe carrier which is quality assured by an effective inspection program and an effective producing steps. Quality of a produced probe carrier is ensured by expanding test items in the processes from synthesis and purification of the probe to fixation thereof to a carrier.

公开(公告)号：EP3086156A1

公开(公告)日：2016-10-26

申请号：EP16165808.3

申请日：2016-04-18

申请人： Canon Kabushiki Kaisha

发明人： MAKARA, Koji ,  OTSUKA, Yoichi ,  KYOGAKU, Masafumi ,  HASHIMOTO, Hiroyuki

IPC分类号： G02B21/00

CPC分类号： G02B21/008 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/65 ,  G01N2021/655 ,  G01N2201/06113 ,  G01N2201/105 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0064 ,  G02B21/0076

摘要： A laser scanning microscope apparatus includes an irradiation unit (1) including an objective lens (13), a photodetector unit (3), an XY-scanning unit (12), and a Z-scanning unit (1, 2, 41). The irradiation unit (1) focuses a laser beam with the objective lens (13) to a specimen (6). The photodetector unit (3) detects light (L) generated from a position (S) irradiated with the laser beam. The XY-scanning unit (12) scans the laser beam in an X-direction perpendicular to an optical axis of the objective lens (13) and in a Y-direction perpendicular to the optical axis and the X-direction. The Z-scanning unit (1, 2, 41) scans the laser beam in a Z-direction parallel to the optical axis. When acquiring XY-two-dimensional image data by detecting the light (L) while scanning the irradiated position (S) in the X-direction and the Y-direction, the apparatus detects the light (L) while scanning the irradiated position (A) also in the Z-direction.

摘要翻译： 激光扫描显微镜装置包括具有物镜（13），光电检测器单元（3），XY扫描单元（12）和Z扫描单元（1,2,3,4）的照射单元（1）。 照射单元（1）将具有物镜（13）的激光束聚焦到样本（6）。 光检测器单元（3）检测从用激光束照射的位置（S）产生的光（L）。 XY扫描单元（12）沿垂直于物镜（13）的光轴的X方向和垂直于光轴和X方向的Y方向扫描激光束。 Z扫描单元（1,2,41）沿与光轴平行的Z方向扫描激光束。 当在X方向和Y方向扫描照射位置（S）时检测光（L）来获取XY-二维图像数据时，该装置在扫描照射位置（A ）也在Z方向。

公开(公告)号：EP2534475A1

公开(公告)日：2012-12-19

申请号：EP11739898.2

申请日：2011-01-31

申请人： Canon Kabushiki Kaisha

发明人： TANJI, Koichi ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC分类号： G01N27/62

CPC分类号： H01J49/0036 ,  H01J49/0004

摘要： A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction).

摘要翻译： 提供了更有效的降噪方法。 在该方法中，当处理具有空间分布的质谱信息时，将整个数据作为三维数据（位置信息存储在xy平面中，并且光谱信息沿z轴方向存储），并且三个 通过对频谱方向和峰值分布方向（面内方向）应用优选的基函数来进行尺寸小波噪声降低。

公开(公告)号：EP2391886A1

公开(公告)日：2011-12-07

申请号：EP10761660.9

申请日：2010-03-30

申请人： Canon Kabushiki Kaisha

发明人： KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC分类号： G01N27/62 ,  G01N33/483 ,  G01N33/68

CPC分类号： G06F19/26 ,  G01N33/6848 ,  H01J49/0004 ,  Y10T436/24

摘要： An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. The present invention provides a method of forming a two-dimensional distribution image of a target constituent based on information on the mass of constituents of the tissue section wherein, as the internal standard material, any one of actin, tubulin and GAPDH is used in the intracellular region, one of histone and nucleic acid is used in the nuclear region, and one of albumin and cytokine is used in the extracellular region.

公开(公告)号：EP1519189A1

公开(公告)日：2005-03-30

申请号：EP03733576.7

申请日：2003-06-26

申请人： CANON KABUSHIKI KAISHA

发明人： OKAMOTO, Tadashi ,  TAKASE, Hiromitsu ,  HASHIMOTO, Hiroyuki

IPC分类号： G01N23/225 ,  G01N33/53

CPC分类号： G01N23/225 ,  C12Q1/6816 ,  C12Q2565/627 ,  C12Q2563/137

摘要： A method of analyzing a measuring sample is provided which is capable of accurately analyzing the state of a probe disposed on a carrier and formation/unformation of a hybrid between the probe and a target nucleic acid, for example, imaging of the disposing locations and quantitative analysis thereof.
The state of a nucleic acid probe formed in a measuring sample obtained by reacting a sample with a probe carrier or formation/unformation of a hybrid between the probe and a target nucleic acid is detected by measurement by the Time-of-Flight Secondary Mass Spectroscopy while being labeled with a marker substance capable of generating fragment ions that are not generated by fragmentation of the probe or the target substance.

摘要翻译： 提供了一种分析测量样品的方法，其能够准确地分析设置在载体上的探针的状态和探针与靶核酸之间的杂交体的形成/变形，例如，将位置的成像和定量 分析。 通过使样品与探针载体反应获得的测量样品中形成的核酸探针的状态或探针和靶核酸之间的杂交体的形成/未形成的状态通过时间 - 飞行二次质谱同时用能够产生碎片离子的标记物质进行标记，该碎片离子不是由探针或靶物质的碎裂产生的。

公开(公告)号：EP2553442A1

公开(公告)日：2013-02-06

申请号：EP11762912.1

申请日：2011-03-28

申请人： Canon Kabushiki Kaisha

发明人： NAITO, Jumpei ,  SATOH, Shuya ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC分类号： G01N27/62 ,  G01N27/64

CPC分类号： G01N33/6851 ,  H01J49/00 ,  Y10T436/182

摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.