发明公开
- 专利标题： Immunoassay reagents and methods of use thereof
- 专利标题（中）： 免疫测定试剂及其使用方法
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申请号： EP11190842.2申请日： 2005-02-24
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公开(公告)号： EP2434286A2公开(公告)日： 2012-03-28
- 发明人: Tacha, David
- 申请人： Biocare Medical, LLC
- 申请人地址： 2940 Camino Diablo Suite 300 Walnut Creek CA 94597 US
- 专利权人： Biocare Medical, LLC
- 当前专利权人： Biocare Medical, LLC
- 当前专利权人地址： 2940 Camino Diablo Suite 300 Walnut Creek CA 94597 US
- 代理机构： Vial, Lionel
- 优先权： US784163 20040224
- 主分类号： G01N33/58
- IPC分类号： G01N33/58 ; G01N1/30 ; G01N33/574 ; G01N33/50
摘要：
The present invention relates to a method of detecting at least two antigens in a sample, comprising:
- contacting the sample with a composition comprising at least one first primary antibody and at least one second primary antibody; wherein the at least one first and second antibodies specifically bind to two antigens selected from p63 and CK5 or P504S, and subsequently
- contacting the sample with a composition comprising at least one first secondary antibody and at least one second secondary antibody, wherein the at least one first secondary antibody is coupled to a poly(alkaline phosphatase) moiety and the at least one second secondary antibody is coupled to a poly(horseradish peroxidase) moiety, and wherein the composition comprises a buffer suitable to stabilize the first and second secondary antibodies; and
- detecting the formation of at least two antigen-antibody complexes on the sample.
- contacting the sample with a composition comprising at least one first primary antibody and at least one second primary antibody; wherein the at least one first and second antibodies specifically bind to two antigens selected from p63 and CK5 or P504S, and subsequently
- contacting the sample with a composition comprising at least one first secondary antibody and at least one second secondary antibody, wherein the at least one first secondary antibody is coupled to a poly(alkaline phosphatase) moiety and the at least one second secondary antibody is coupled to a poly(horseradish peroxidase) moiety, and wherein the composition comprises a buffer suitable to stabilize the first and second secondary antibodies; and
- detecting the formation of at least two antigen-antibody complexes on the sample.
公开/授权文献
- EP2434286A3 Immunoassay reagents and methods of use thereof 公开/授权日：2012-06-13
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