公开(公告)号：EP2434286A2

公开(公告)日：2012-03-28

申请号：EP11190842.2

申请日：2005-02-24

申请人： Biocare Medical, LLC

发明人： Tacha, David

IPC分类号： G01N33/58 ,  G01N1/30 ,  G01N33/574 ,  G01N33/50

CPC分类号： G01N33/581 ,  G01N1/30 ,  G01N33/5082 ,  G01N33/5091 ,  G01N33/57484 ,  G01N33/57492

摘要： The present invention relates to a method of detecting at least two antigens in a sample, comprising:
- contacting the sample with a composition comprising at least one first primary antibody and at least one second primary antibody; wherein the at least one first and second antibodies specifically bind to two antigens selected from p63 and CK5 or P504S, and subsequently
- contacting the sample with a composition comprising at least one first secondary antibody and at least one second secondary antibody, wherein the at least one first secondary antibody is coupled to a poly(alkaline phosphatase) moiety and the at least one second secondary antibody is coupled to a poly(horseradish peroxidase) moiety, and wherein the composition comprises a buffer suitable to stabilize the first and second secondary antibodies; and
- detecting the formation of at least two antigen-antibody complexes on the sample.

摘要翻译： 本发明涉及检测样品中至少两种抗原的方法，所述方法包括： - 使所述样品与包含至少一种第一一抗和至少一种第二一抗的组合物接触; 其中所述至少一种第一和第二抗体特异性结合选自p63和CK5或P504S的两种抗原，并且随后 - 使所述样品与包含至少一种第一次级抗体和至少一种第二次级抗体的组合物接触，其中所述至少一种第一和第二抗体 一个第一次级抗体偶联于聚（碱性磷酸酶）部分，并且所述至少一个第二次级抗体偶联于聚（辣根过氧化物酶）部分，并且其中所述组合物包含适于稳定所述第一和第二次级抗体的缓冲液; 和 - 检测样品上至少两种抗原 - 抗体复合物的形成。

公开(公告)号：EP2818869A1

公开(公告)日：2014-12-31

申请号：EP14178215.1

申请日：2005-02-24

申请人： Biocare Medical, LLC

发明人： Tacha, David

IPC分类号： G01N33/58 ,  G01N1/30 ,  G01N33/574 ,  G01N33/50

CPC分类号： G01N33/581 ,  G01N1/30 ,  G01N33/5082 ,  G01N33/5091 ,  G01N33/57484 ,  G01N33/57492

摘要： The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and efficiently.

摘要翻译： 本发明提供使用试剂的试剂和方法，例如在自动染色装置上，便于简单有效地检测样品中两种或更多种抗原。

公开(公告)号：EP2434286A3

公开(公告)日：2012-06-13

申请号：EP11190842.2

申请日：2005-02-24

申请人： Biocare Medical, LLC

发明人： Tacha, David

IPC分类号： G01N33/58 ,  G01N1/30 ,  G01N33/574 ,  G01N33/50

CPC分类号： G01N33/581 ,  G01N1/30 ,  G01N33/5082 ,  G01N33/5091 ,  G01N33/57484 ,  G01N33/57492

摘要： The present invention relates to a method of detecting at least two antigens in a sample, comprising:
- contacting the sample with a composition comprising at least one first primary antibody and at least one second primary antibody; wherein the at least one first and second antibodies specifically bind to two antigens selected from p63 and CK5 or P504S, and subsequently
- contacting the sample with a composition comprising at least one first secondary antibody and at least one second secondary antibody, wherein the at least one first secondary antibody is coupled to a poly(alkaline phosphatase) moiety and the at least one second secondary antibody is coupled to a poly(horseradish peroxidase) moiety, and wherein the composition comprises a buffer suitable to stabilize the first and second secondary antibodies; and
- detecting the formation of at least two antigen-antibody complexes on the sample.