发明公开
EP2463366A9 NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND
有权
新的糖基转移酶,新的糖基转移酶基因和新的糖基化合物供体化合物
- 专利标题: NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND
- 专利标题(中): 新的糖基转移酶,新的糖基转移酶基因和新的糖基化合物供体化合物
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申请号: EP10806260.5申请日: 2010-01-21
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公开(公告)号: EP2463366A9公开(公告)日: 2013-01-16
- 发明人: OZEKI Yoshihiro , SASAKI Nobuhiro , NAGASAWA Kazuo , TERA Masayuki , MATSUBA Yuki , NAKAMURA Haruka , ABE Yutaka
- 申请人: National University Corporation Tokyo University Of Agriculture and Technology
- 申请人地址: 3-8-1 Harumi-cho Fuchu-shi Tokyo 183-8538 JP
- 专利权人: National University Corporation Tokyo University Of Agriculture and Technology
- 当前专利权人: National University Corporation Tokyo University Of Agriculture and Technology
- 当前专利权人地址: 3-8-1 Harumi-cho Fuchu-shi Tokyo 183-8538 JP
- 代理机构: Schrell, Andreas
- 优先权: JP2009184030 20090807
- 国际公布: WO2011016260 20110210
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C07H13/08 ; C07H17/065 ; C12P19/44
摘要:
An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A):
wherein R 1 is independently selected from hydrogen, or C 1-6 alkyl, C 2-6 alkenyl, and C 2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO 2 , and SO 2 , n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.
wherein R 1 is independently selected from hydrogen, or C 1-6 alkyl, C 2-6 alkenyl, and C 2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO 2 , and SO 2 , n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.
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