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    NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND
    1.
    发明公开
    NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND 有权
    神经糖蛋白酶，神经胶原蛋白

    公开(公告)号：EP2463366A1

    公开(公告)日：2012-06-13

    申请号：EP10806260.5

    申请日：2010-01-21

    申请人： National University Corporation Tokyo University Of Agriculture and Technology

    发明人： OZEKI Yoshihiro ,  SASAKI Nobuhiro ,  NAGASAWA Kazuo ,  TERA Masayuki ,  MATSUBA Yuki ,  NAKAMURA Haruka ,  ABE Yutaka

    IPC分类号： C12N15/09 ,  C07H13/08 ,  C07H17/065 ,  C12P19/44

    CPC分类号： C12N9/1048 ,  C07H13/08 ,  C07H15/18 ,  C07H17/065 ,  C12P19/44 ,  C12P19/60

    摘要： An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A):

    wherein R 1 is independently selected from hydrogen, or C 1-6 alkyl, C 2-6 alkenyl, and C 2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO 2 , and SO 2 , n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

    摘要翻译： 本发明的目的是提供一种含糖糖核苷酸以外的糖供体化合物和能够使用除了糖核苷酸以外的糖供体化合物来催化糖基转移反应的酶的供糖试剂。 本发明提供以下：含有式（A）化合物的给糖试剂：其中R 1独立地选自氢或C 1-6烷基，C 2-6烯基和C 2-6炔基，其中 每个基团是未取代的或被一个或多个选自OH，F，Cl，Br，I，CN，NO 2和SO 2的基团取代，n是0,1,2,3,4或5，m是 0或1，X表示通过其端基异碳上的2键键合的单糖; 能够使用糖供体催化糖基转移反应的糖基转移酶; 和包含编码糖基转移酶的DNA的糖基转移酶基因。

    NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND
    2.
    发明公开
    NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND 有权
    新的糖基转移酶，新的糖基转移酶基因和新的糖基化合物供体化合物

    公开(公告)号：EP2463366A9

    公开(公告)日：2013-01-16

    申请号：EP10806260.5

    申请日：2010-01-21

    申请人： National University Corporation Tokyo University Of Agriculture and Technology

    发明人： OZEKI Yoshihiro ,  SASAKI Nobuhiro ,  NAGASAWA Kazuo ,  TERA Masayuki ,  MATSUBA Yuki ,  NAKAMURA Haruka ,  ABE Yutaka

    IPC分类号： C12N15/09 ,  C07H13/08 ,  C07H17/065 ,  C12P19/44

    CPC分类号： C12N9/1048 ,  C07H13/08 ,  C07H15/18 ,  C07H17/065 ,  C12P19/44 ,  C12P19/60

    摘要： An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A):

    wherein R 1 is independently selected from hydrogen, or C 1-6 alkyl, C 2-6 alkenyl, and C 2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO 2 , and SO 2 , n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

    摘要翻译： 本发明的目的在于提供包含糖核苷酸以外的糖供体化合物和能够使用糖核苷酸以外的糖供体化合物来催化糖基转移反应的酶的供糖试剂。 本发明提供如下：含有式（A）化合物的供糖试剂：其中R 1独立地选自氢或C 1-6烷基，C 2-6烯基和C 2-6炔基，其中每个基团 未被取代或被一个或多个选自OH，F，Cl，Br，I，CN，NO 2和SO 2的基团取代，n为0,1,2,3,4或5，m为0或1，并且X 表示通过其异头碳上的β键结合的单糖; 使用糖供体能够催化糖基转移反应的糖基转移酶; 和包含编码糖基转移酶的DNA的糖基转移酶基因。