发明公开
- 专利标题： INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDES
- 专利标题（中）： N-甲基赖氨酸的INSET多肽中
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申请号： EP10777063.8申请日： 2010-10-01
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公开(公告)号： EP2483397A2公开(公告)日： 2012-08-08
- 发明人: CHIN, Jason , NGUYEN, Duy, P.
- 申请人： Medical Research Council
- 申请人地址： 20 Park Crescent London W1B 1AL GB
- 专利权人： Medical Research Council
- 当前专利权人： Medical Research Council
- 当前专利权人地址： 20 Park Crescent London W1B 1AL GB
- 代理机构： Richards, William John
- 优先权： GB0917240 20091001
- 国际公布： WO2011039518 20110407
- 主分类号： C12N9/00
- IPC分类号： C12N9/00
摘要：
The invention relates to A method of making a polypeptide comprising at least one N
ε -methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R-N
ε -methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R- Nε-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R-N
ε -methyl-lysine.
ε -methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R-N
ε -methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R- Nε-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R-N
ε -methyl-lysine.
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