发明公开
EP2484757A9 VIRUS CAPABLE OF EXPRESSING -GALACTOSE EPITOPE, AND METHOD FOR PRODUCTION OF VACCINE
有权
病毒能够表达β-半乳糖表位的疫苗和生产疫苗的方法
- 专利标题： VIRUS CAPABLE OF EXPRESSING -GALACTOSE EPITOPE, AND METHOD FOR PRODUCTION OF VACCINE
- 专利标题（中）： 病毒能够表达β-半乳糖表位的疫苗和生产疫苗的方法
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申请号： EP10820641.8申请日： 2010-09-30
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公开(公告)号： EP2484757A9公开(公告)日： 2013-12-04
- 发明人: OGAWA, Haruko , IMAI, Kunitoshi , TAGAMI, Takahiro
- 申请人： National University Corporation Obihiro University of Agriculture and Veterinary Medicine , Incorporated Administrative Agency National Agriculture and Food Research Organization
- 申请人地址： 11, Nishi 2-sen, Inada-cho Obihiro-shi, Hokkaido 080-8555 JP
- 专利权人： National University Corporation Obihiro University of Agriculture and Veterinary Medicine,Incorporated Administrative Agency National Agriculture and Food Research Organization
- 当前专利权人： National University Corporation Obihiro University of Agriculture and Veterinary Medicine,Incorporated Administrative Agency National Agriculture and Food Research Organization
- 当前专利权人地址： 11, Nishi 2-sen, Inada-cho Obihiro-shi, Hokkaido 080-8555 JP
- 代理机构： Vossius & Partner
- 优先权： JP2009227753 20090930
- 国际公布： WO2011040526 20110407
- 主分类号： C12N7/00
- IPC分类号： C12N7/00 ; A61K39/145 ; A61K39/165 ; A61K39/17 ; A61K39/20 ; A61K39/21 ; A61K39/255 ; A61K39/285 ; A61P31/12 ; A61P31/16 ; C12N7/04 ; C12N15/09 ; C12N9/10
摘要：
A method for producing an α-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an α-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an α-galactose epitope (Galα1-3Galβ1-4GlcNAc -R: α-Gal hereinafter)-expressing virus, which comprises the steps of:
(1) introducing an α1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express α-Gal to obtain a cell line capable of expressing α-Gal;
(2) inoculating a virus into the cell line capable of expressing α-Gal to obtain a cell line infected with a virus; and
(3) culturing the cell line infected with the virus to obtain a virus expressing α-Gal from the culture medium.
(1) introducing an α1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express α-Gal to obtain a cell line capable of expressing α-Gal;
(2) inoculating a virus into the cell line capable of expressing α-Gal to obtain a cell line infected with a virus; and
(3) culturing the cell line infected with the virus to obtain a virus expressing α-Gal from the culture medium.
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