公开(公告)号：EP2484757A9

公开(公告)日：2013-12-04

申请号：EP10820641.8

申请日：2010-09-30

申请人： National University Corporation Obihiro University of Agriculture and Veterinary Medicine ,  Incorporated Administrative Agency National Agriculture and Food Research Organization

发明人： OGAWA, Haruko ,  IMAI, Kunitoshi ,  TAGAMI, Takahiro

IPC分类号： C12N7/00 ,  A61K39/145 ,  A61K39/165 ,  A61K39/17 ,  A61K39/20 ,  A61K39/21 ,  A61K39/255 ,  A61K39/285 ,  A61P31/12 ,  A61P31/16 ,  C12N7/04 ,  C12N15/09 ,  C12N9/10

CPC分类号： A61K39/145 ,  A61K39/00 ,  A61K39/12 ,  C12N7/00 ,  C12N9/1051 ,  C12N2760/16034 ,  C12N2760/16052

摘要： A method for producing an α-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an α-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an α-galactose epitope (Galα1-3Galβ1-4GlcNAc -R: α-Gal hereinafter)-expressing virus, which comprises the steps of:
(1) introducing an α1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express α-Gal to obtain a cell line capable of expressing α-Gal;
(2) inoculating a virus into the cell line capable of expressing α-Gal to obtain a cell line infected with a virus; and
(3) culturing the cell line infected with the virus to obtain a virus expressing α-Gal from the culture medium.

摘要翻译： 一种生产表达α-Gal的病毒的方法，所述病毒对病毒具有增强的免疫应答，而不需要使用任何酶; 具有高效果（抗原性）的流感病毒疫苗，其使用通过该方法产生的表达α-Gal的病毒产生; 和别的。 具体而言，公开了下述α-半乳糖表位（Galα1-3Galβ1-4GlcNAc-R：α-Gal）表达病毒的制造方法，其包括以下步骤：（1）将α1,3-半乳糖基转移酶基因导入 可表达条件转化到不表达α-Gal的细胞系中以获得能够表达α-Gal的细胞系; （2）将病毒接种到能够表达α-Gal的细胞系中以获得感染病毒的细胞系; 和（3）培养感染该病毒的细胞系以从培养基中获得表达α-Gal的病毒。

公开(公告)号：EP2484757A1

公开(公告)日：2012-08-08

申请号：EP10820641.8

申请日：2010-09-30

申请人： National University Corporation Obihiro University of Agriculture and Veterinary Medicine ,  Incorporated Administrative Agency National Agriculture and Food Research Organization

发明人： OGAWA, Haruko ,  IMAI, Kunitoshi ,  TAGAMI, Takahiro

IPC分类号： C12N7/00 ,  A61K39/145 ,  A61K39/165 ,  A61K39/17 ,  A61K39/20 ,  A61K39/21 ,  A61K39/255 ,  A61K39/285 ,  A61P31/12 ,  A61P31/16 ,  C12N7/04 ,  C12N15/09

CPC分类号： A61K39/145 ,  A61K39/00 ,  A61K39/12 ,  C12N7/00 ,  C12N9/1051 ,  C12N2760/16034 ,  C12N2760/16052

摘要： A method for producing an α-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an α-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an α-galactose epitope (Galα1-3Galβ1-4GlcNAc -R: α-Gal hereinafter)-expressing virus, which comprises the steps of:
(1) introducing an α1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express α-Gal to obtain a cell line capable of expressing α-Gal;
(2) inoculating a virus into the cell line capable of expressing α-Gal to obtain a cell line infected with a virus; and
(3) culturing the cell line infected with the virus to obtain a virus expressing α-Gal from the culture medium.

摘要翻译： 一种用于产生对病毒具有增强免疫应答的±-Gal表达病毒的方法，而不需要使用任何酶; 使用通过该方法生产的±-Gal表达病毒产生的具有高效应（抗原性）的流感病毒疫苗; 和别的。 具体公开的是：产生表达表达的病毒的半乳糖表位（Gal±1-3Gal21-4GlcNAc -R：±-Gal）的方法，其包括以下步骤：（1）引入±1,3-半乳糖基转移酶 基因在可表达条件下进入不表达±-Gal的细胞系以获得能够表达±-Gal的细胞系; （2）将病毒接种到能够表达±-Gal的细胞系中以获得感染病毒的细胞系; （3）培养感染病毒的细胞系，从培养基中获得表达±-Gal的病毒。

公开(公告)号：EP2484757B1

公开(公告)日：2015-04-29

申请号：EP10820641.8

申请日：2010-09-30

申请人： National University Corporation Obihiro University of Agriculture and Veterinary Medicine

发明人： OGAWA, Haruko ,  IMAI, Kunitoshi ,  TAGAMI, Takahiro

IPC分类号： C12N7/00 ,  A61K39/145 ,  A61K39/165 ,  A61K39/17 ,  A61K39/20 ,  A61K39/21 ,  A61K39/255 ,  A61K39/285 ,  A61P31/12 ,  A61P31/16 ,  C12N7/04 ,  C12N15/09 ,  C12N9/10

CPC分类号： A61K39/145 ,  A61K39/00 ,  A61K39/12 ,  C12N7/00 ,  C12N9/1051 ,  C12N2760/16034 ,  C12N2760/16052