发明公开
EP2511293A1 A method for controlling the main complex N-glycan structures and the acidic variants and variability in bioprocesses producing recombinant proteins
审中-公开
用于控制主复合N-聚糖结构和酸变体和变异的生物工艺生产重组蛋白的方法
- 专利标题： A method for controlling the main complex N-glycan structures and the acidic variants and variability in bioprocesses producing recombinant proteins
- 专利标题（中）： 用于控制主复合N-聚糖结构和酸变体和变异的生物工艺生产重组蛋白的方法
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申请号： EP11162193.4申请日： 2011-04-13
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公开(公告)号： EP2511293A1公开(公告)日： 2012-10-17
- 发明人: Stojkovic, Sasa , Spudic, Vatroslav , Tisu, Matjaz , Koncilja, Matjaz
- 申请人： LEK Pharmaceuticals d.d.
- 申请人地址： Verovskova 57 1526 Ljubljana SI
- 专利权人： LEK Pharmaceuticals d.d.
- 当前专利权人： LEK Pharmaceuticals d.d.
- 当前专利权人地址： Verovskova 57 1526 Ljubljana SI
- 代理机构： Dehmel, Albrecht
- 主分类号： C07K14/435
- IPC分类号： C07K14/435 ; C12N5/00 ; C07K14/43
摘要：
The present invention relates to a method of controlling quality and quantity of posttranslational modification of a recombinantly produced polypeptide/protein (glycoprotein), wherein the posttranslational modification affects the glycosylation profile and/or the acidic variants profile, as manifested in CEX profiles, wherein the polypeptide/protein (glycoprotein) production is in eukaryotic host cells, the method comprising the following steps:
a) cultivating the eukaryotic cells in a suitable medium under conditions which allow the expression of the polypeptide/protein, wherein the content of the dissolved CO 2 (pCO 2 ) in the medium is at a first value during the initial growth phase of the eukaryotic cells, allowing the eukaryotic cells to grow, and
b) increasing or decreasing the content of the dissolved CO 2 (pCO 2 ) in the medium during the production phase of the eukaryotic cells to a second value.
a) cultivating the eukaryotic cells in a suitable medium under conditions which allow the expression of the polypeptide/protein, wherein the content of the dissolved CO 2 (pCO 2 ) in the medium is at a first value during the initial growth phase of the eukaryotic cells, allowing the eukaryotic cells to grow, and
b) increasing or decreasing the content of the dissolved CO 2 (pCO 2 ) in the medium during the production phase of the eukaryotic cells to a second value.
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