发明公开
EP2516637A1 THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS
有权
用于核苷酸合成耐热Biokatalysatorkombination
- 专利标题： THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS
- 专利标题（中）： 用于核苷酸合成耐热Biokatalysatorkombination
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申请号： EP10798096.3申请日： 2010-12-22
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公开(公告)号： EP2516637A1公开(公告)日： 2012-10-31
- 发明人: MONTILLA AREVALO, Rafael , DERONCELÉ THOMAS, Víctor, Manuel , LÓPEZ GÓMEZ, Cristina , PASCUAL GILABERT, Marta , ESTÉVEZ COMPANY, Carlos , CASTELLS BOLIART, Josep
- 申请人： Plasmia Biotech, S.L.
- 申请人地址： C. Álvarez de Castro, 63 08100 Mollet del Vallès ES
- 专利权人： Plasmia Biotech, S.L.
- 当前专利权人： Plasmia Biotech, S.L.
- 当前专利权人地址： C. Álvarez de Castro, 63 08100 Mollet del Vallès ES
- 代理机构： Ponti Sales, Adelaida
- 优先权： EP09382296 20091222
- 国际公布： WO2011076894 20110630
- 主分类号： C12N9/10
- IPC分类号： C12N9/10 ; C12P19/38
摘要：
The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from
Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from
Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of
Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of
Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from
Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of
Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of
Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
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