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1.发明授权
公开(公告)号：EP2516637B1
公开(公告)日：2016-04-20
申请号：EP10798096.3
申请日：2010-12-22
发明人： MONTILLA AREVALO, Rafael , DERONCELÉ THOMAS, Víctor, Manuel , LÓPEZ GÓMEZ, Cristina , PASCUAL GILABERT, Marta , ESTÉVEZ COMPANY, Carlos , CASTELLS BOLIART, Josep
CPC分类号： C12N9/1077 , C12P19/38
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2.发明公开
公开(公告)号：EP2516637A1
公开(公告)日：2012-10-31
申请号：EP10798096.3
申请日：2010-12-22
发明人： MONTILLA AREVALO, Rafael , DERONCELÉ THOMAS, Víctor, Manuel , LÓPEZ GÓMEZ, Cristina , PASCUAL GILABERT, Marta , ESTÉVEZ COMPANY, Carlos , CASTELLS BOLIART, Josep
CPC分类号： C12N9/1077 , C12P19/38
摘要： The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from
Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from
Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of
Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of
Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
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