Aqueous glyphosate formulations comprising a surfactant derived from metathesized natural oil feedstocks are disclosed. The formulations comprise a glyphosate salt, water, and a surfactant derived from a metathesis-derived C
10 -C
17 monounsaturated acid, octadecene-1,18-dioic acid, or their ester derivatives. The surfactant is selected from C
10 or C
12 amine oxides, C
10 or C
12 quats, C
10 , C
12 , or C
16 amidoamines, C
10 or C
12 amidoamine oxides, C
10 imidazoline quats, C
10 or C
12 amidoamine quats, C
10 , C
12 , or C
16 betaines, C
16 amidoamine betaines, C
18 diamidoamines, C
18 diamidoamine oxides, C
18 diamidoamine diquats, C
18 diamidoamine oxide quats, C
18 diamidoamine oxide betaines, Cis diamidoamine monobetaines, C
18 diamidoamine monobetaine quats, C
18 ester amidoamine quats, and amidoamines and their oxidized or quaternized derivatives made from self- or cross-metathesized palm or soybean oil. The surfactants noted above impart substantial stability to highly concentrated glyphosate formulations at, above, and below room temperature and perform as well or better than commercial alternatives.