A device for receiving or providing a fluid and a method of selectively coupling a tube (22) to the device with the tube receiving or providing the fluid. The device includes a housing (24) and a female port (26) extending from the housing. The female port has an engagement surface (28) and defines an interior chamber ( 30 ) with a retainer (62) disposed in the interior chamber. The retainer has a base (64), a finger (66) extending from the base to selectively engage the engagement surface, and a barb (88) proximate to the finger for selectively engaging the tube. A seal (52) is disposed in the interior chamber of the female port adjacent the retainer. The retainer (90) has a stud extending from the base beyond the barb to engage the seal for biasing the retainer away from the housing to facilitate movement of the finger against the engagement surface and engagement of the tube with the barb.