发明公开
EP2642286A1 NOVEL METHOD FOR ANALYZING GLYCOSAMINOGLYCAN
审中-公开
VERFAHREN ZUR ANALYZE VON GLYKOSAMINOGLYKAN
- 专利标题： NOVEL METHOD FOR ANALYZING GLYCOSAMINOGLYCAN
- 专利标题（中）： VERFAHREN ZUR ANALYZE VON GLYKOSAMINOGLYKAN
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申请号： EP11841620.5申请日： 2011-11-18
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公开(公告)号： EP2642286A1公开(公告)日： 2013-09-25
- 发明人: TAKEGAWA, Yasuhiro , SHINOHARA, Yasuro , SAKAI, Hideaki
- 申请人： CellSeed Inc. , National University Corporation Hokkaido University
- 申请人地址： 33-8, Wakamatsu-cho Shinjuku-ku Tokyo 162-0056 JP
- 专利权人： CellSeed Inc.,National University Corporation Hokkaido University
- 当前专利权人： CellSeed Inc.,National University Corporation Hokkaido University
- 当前专利权人地址： 33-8, Wakamatsu-cho Shinjuku-ku Tokyo 162-0056 JP
- 代理机构： HOFFMANN EITLE
- 优先权： JP2010258385 20101118
- 国际公布： WO2012067236 20120524
- 主分类号： G01N30/88
- IPC分类号： G01N30/88 ; G01N27/62 ; G01N30/06 ; G01N30/72 ; G01N30/74
摘要：
A method of analyzing a sugar chain by high performance liquid chromatography uses a column packed with a stationary phase including anion and cation exchangers. A fluorescence-labeled sample is prepared for the analysis by decomposing glycosaminoglycans to be analyzed into their constitutional units being disaccharides, capturing the disaccharides by glycoblotting, releasing the disaccharides by acid treatment, and subsequently reductively aminating the released disaccharides. In this method, two or more types of glycosaminoglycans can be simultaneously analyzed.
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